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Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot
1Department of Horticulture, 1575 Linden Drive, University of Wisconsin, Madison, WI 53706, USA, , , , , , US.
Abstract:
Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones `DDR7099', `PI383819', and `Piacenza' were utilized as an explant source for continuous, friable callus production. The best callus production occurred on root segments initially cultured on medium with 4,5 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 weeks, then subcultured to medium with 4.7 μM 4-amino-3,5,6-trichloropicolinic acid (picloram) +0.49 μM 6-(γ-γ-dimethylallylamino)purine (2iP) for 8 weeks. Embryogenic, friable callus was transferred to liquid medium for 1 month and then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively) occurred on 4-month-old calli from the clone `DDR7099'. In all clones, regeneration rate decreased as callus age increased.
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