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Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
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Silver nanoparticles inhibit beige fat function and promote adiposity.

Lishu Yue1, Wenjun Zhao1, Dongmei Wang1

  • 1Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Shanghai, 200241, China.

Molecular Metabolism
|February 10, 2019
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Summary

Silver nanoparticles (AgNPs) exposure unexpectedly promotes obesity by inhibiting beige adipocyte function and thermogenesis. This occurs through increased oxidative stress and MAPK-ERK signaling, highlighting potential health risks of AgNPs.

Keywords:
AdiposityBeige fatBrowningEnergy expenditureReactive oxidative speciesSilver nanoparticles

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Area of Science:

  • Environmental Science
  • Toxicology
  • Metabolic Disease

Background:

  • Obesity is a global health crisis with complex causes.
  • Environmental pollutants are increasingly implicated in metabolic dysfunction.
  • Silver nanoparticles (AgNPs) are ubiquitous, but their role in obesity is unknown.

Purpose of the Study:

  • To investigate the impact of AgNPs on beige adipocyte differentiation and function.
  • To assess AgNPs' effects on adiposity and metabolism in mice.
  • To elucidate the molecular mechanisms underlying AgNPs' influence on beige adipocytes.

Main Methods:

  • In vitro studies on beige adipocyte differentiation and functionality.
  • In vivo experiments in mice to evaluate AgNPs' effect on adiposity and metabolic performance.
  • Molecular analysis of gene expression, reactive oxygen species (ROS), and signaling pathways (MAPK-ERK).

Main Results:

  • AgNPs suppressed beige adipocyte differentiation, mitochondrial activity, and thermogenic gene programs.
  • In mice, AgNPs exposure reduced energy expenditure and increased adiposity by inhibiting subcutaneous fat browning.
  • AgNPs increased ROS levels and activated MAPK-ERK signaling in beige adipocytes.

Conclusions:

  • AgNPs promote adiposity by inhibiting beige adipocyte differentiation and function, likely via ROS and ERK pathways.
  • These findings reveal an unexpected role for AgNPs in obesity development.
  • Further research is needed to assess AgNPs' health risks and safe usage levels.