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Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
Published on: June 23, 2018
Detection and Elimination of Cellular Bottlenecks in Protein-Producing Yeasts
Richard J Zahrl1,2, Brigitte Gasser3,4, Diethard Mattanovich4
1Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria.
Abstract:
Yeasts are efficient cell factories and are commonly used for the production of recombinant proteins for biopharmaceutical and industrial purposes. For such products high levels of correctly folded proteins are needed, which sometimes requires improvement and engineering of the expression system. The article summarizes major breakthroughs that led to the efficient use of yeasts as production platforms and reviews bottlenecks occurring during protein production. Special focus is given to the metabolic impact of protein production. Furthermore, strategies that were shown to enhance secretion of recombinant proteins in different yeast species are presented.
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