Related Experiment Videos
Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems
1New England Biolabs, Inc., Beverly, MA 01915.
Gene
|December 25, 1988
Abstract:
Methyltransferase genes from the FnuDI, NaeI, NcoI, and XbaI restriction-modification systems have been isolated in Escherichia coli by 'shot-gun' cloning bacterial DNA fragments into plasmid vectors and selecting for protectively modified molecules that resist digestion by the corresponding restriction endonuclease.