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Related Concept Videos

Kidney Structure01:45

Kidney Structure

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The kidneys are two large bean-shaped organs located in the upper abdomen. They filter the blood several times a day to remove toxins and rebalance water and electrolytes of the circulatory system via the renal veins. The kidneys receive blood directly from the heart via the renal arteries. These arteries enter the kidney at the hilum, the concave surface of the bean, where they branch and divide into smaller vessels and capillaries.
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Adult Stem Cells01:33

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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External Anatomy of the Kidney01:21

External Anatomy of the Kidney

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The kidneys are a pair of bean-shaped organs in the human body that play a critical role in maintaining overall health. They filter out waste products from the blood, regulate blood pressure, maintain electrolyte balance, and stimulate the production of red blood cells.
The kidneys are located in the retroperitoneal space on either side of the vertebral column, protected posteriorly by the 11th and 12th ribs. The right kidney sits slightly lower than the left owing to the presence of the liver...
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Internal Anatomy of the Kidney01:12

Internal Anatomy of the Kidney

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The kidneys are essential organs in the human body, performing a myriad of tasks that maintain homeostasis and overall health.
Anatomical Position and Dimensions
The kidneys are retroperitoneal organs positioned against the posterior abdominal wall on either side of the spine, roughly between the twelfth thoracic and third lumbar vertebrae. Each kidney is typically 10-12 cm long, 5-6 cm wide, and 3-4 cm thick, weighing about 150 grams.
Renal Cortex
The outermost region of the kidney is the...
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Kidney Transplant I: Introduction01:28

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A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
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Anatomy of the Genitourinary System I: Kidneys and Ureters01:11

Anatomy of the Genitourinary System I: Kidneys and Ureters

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The upper urinary system comprises two kidneys and two ureters, which are crucial in filtering blood and forming urine.KidneysLocation and Structure:The kidneys are two bean-shaped organs positioned behind the peritoneum on either side of the spine.Kidneys are between the 12th thoracic (T12) and the 3rd lumbar (L3) vertebrae.The position of the liver causes the right kidney to sit slightly lower than the left.Protective Layers:Each kidney is enveloped in a tough, fibrous membrane called the...
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Related Experiment Video

Updated: Jan 29, 2026

Decellularization and Recellularization of Whole Livers
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Decellularizing and Recellularizing Adult Mouse Kidneys.

Theresa Chow1,2, Jennifer Whiteley1, Ian M Rogers3,4,5

  • 1Lunenfeld-Tanenbaum Research Institute, Mt. Sinai Hospital, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|February 12, 2019
PubMed
Summary
This summary is machine-generated.

This study details decellularization methods for mouse kidneys using sodium dodecyl sulfate (SDS). Researchers also explored strategies for repopulating these decellularized kidney scaffolds with cells for tissue engineering applications.

Keywords:
BioreactorCannulation and perfusionExtracellular matrixKidney decellularizationKidney recellularizationMouse kidney dissectionThree-dimensional (3D) tissue culture

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Decellularization removes cells from tissues, preserving the extracellular matrix (ECM).
  • ECM scaffolds are crucial for three-dimensional cell culture and tissue regeneration.
  • Developing effective decellularization protocols is key for creating viable tissue constructs.

Purpose of the Study:

  • To establish methods for decellularizing whole and thick mouse kidney sections.
  • To investigate strategies for repopulating decellularized kidney scaffolds with cells.
  • To advance the use of decellularized ECM in regenerative medicine.

Main Methods:

  • Decellularization of mouse kidneys using 0.1% sodium dodecyl sulfate (SDS) detergent solution.
  • Processing of both whole and thick kidney sections.
  • Development of cell repopulation techniques for the decellularized scaffolds.

Main Results:

  • Successful decellularization of mouse kidney tissue was achieved.
  • The integrity of the extracellular matrix scaffold was maintained post-decellularization.
  • Methods for effective cell seeding and growth within the decellularized scaffolds were explored.

Conclusions:

  • Decellularized mouse kidneys provide a viable scaffold for cell culture.
  • This technique supports the development of kidney tissue engineering strategies.
  • Further research can optimize repopulation for potential therapeutic applications.