Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Self-Locking Screw01:16

Self-Locking Screw

2.5K
A square-threaded screw jack is a mechanical device widely used for lifting heavy loads or applying considerable force. One of the key features that can make a screw jack more effective and reliable is its self-locking capability.
A square-threaded screw jack carrying a load is considered self-locking if the screw retains its position even after the moment applied to it is removed.
2.5K
Interpreting R Charts01:22

Interpreting R Charts

348
R chart, or range chart, is a fundamental tool in statistical process control used to monitor the variability within a process. It complements the X-bar (x̄) chart by focusing on the range of the data, rather than individual values, providing a clear picture of the process dispersion over time.
An R chart plots the range of subsets of measurements collected from a process. Each point on the chart represents the range—defined as the difference between the maximum and minimum...
348
Interpreting Run Charts01:25

Interpreting Run Charts

3.3K
Run charts, essentially line graphs plotted over time, serve as fundamental yet effective tools for process analysis. They chronicle data sequentially, facilitating the identification of trends, shifts, or cyclical movements. This graphical representation is instrumental in determining whether a process is stable or exhibits signs of potential instability indicative of special cause variation. In the healthcare domain, run charts depict infection rates over time, enabling hospitals to monitor...
3.3K
Interpretation of Confidence Intervals01:19

Interpretation of Confidence Intervals

10.0K
A confidence interval is a better estimate of the population than a point estimate, as it uses a range of values from a sample instead of a single value.
Confidence intervals have confidence coefficients that are crucial for their interpretation. The most common confidence coefficients are 0.90, 0.95, and 0.99, which can be written as percentages–90%, 95%, and 99%, respectively.
Suppose a person calculates a confidence interval with a confidence coefficient of 0.95. In that case, they can...
10.0K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

3.3K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
3.3K
Kinematic Equations for Rotation01:30

Kinematic Equations for Rotation

807
In mechanics, when one observes a rigid body in rotational motion with constant angular acceleration, it is possible to establish equations for its rotational kinematics. This process resembles how linear kinematics are dealt with in simpler motion studies.
For instance, imagine a point A on a rigid body engaged in circular motion. The translational velocity of this particular point can be calculated by taking the time derivatives of the displacement equation, which essentially measures the...
807

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A distinct E dimer epitope underlies selective recognition by a protective human West Nile virus antibody.

EMBO reports·2026
Same author

[A CBCT study on the inclination of the maxillary occlusal plane and related measurements in adolescent skeletal Classâ… and Classâ…¡patients with normal vertical angles].

Shanghai kou qiang yi xue = Shanghai journal of stomatology·2026
Same author

In situ structures of the portal-neck-tail complex of bacteriophage T4 inform a viral genome positioning mechanism.

Nature communications·2026
Same author

Cryo-EM structures of bacteriophage T4 portal-neck assembly intermediates reveal a viral genome retention mechanism.

Nature communications·2026
Same author

Structure of human mitochondrial pyruvate carrier MPC1 and MPC2 complex.

Nature communications·2025
Same author

Cryo-EM structures of bacteriophage T4 portal-neck connector complexes reveal a viral genome retention mechanism involving a host component.

Research square·2025
Same journal

Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus.

Journal of structural biology·2026
Same journal

Architecture and dynamics of a supramolecular oxygen transport system in human homogentisate 1,2-Dioxygenase.

Journal of structural biology·2026
Same journal

Connecting pathways between mineralized fibrocartilage and bone at the Achilles tendon insertion.

Journal of structural biology·2026
Same journal

Structural and functional characterization of thermostable EstS1 esterase for BHET degradation.

Journal of structural biology·2026
Same journal

Following the white rabbit: multiscale 2D3D correlative imaging of bone structure.

Journal of structural biology·2026
Same journal

The mantis shrimp eye imaged in 3D using 4th generation synchrotron multiscale phase contrast tomography.

Journal of structural biology·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
14:18

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements

Published on: February 28, 2016

11.9K

Enhancement of tomogram interpretability using the locked self-rotation function.

Andrei Fokine1, Baldeep Khare1, Yingyuan Sun1

  • 1Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, United States.

Journal of Structural Biology
|February 12, 2019
PubMed
Summary
This summary is machine-generated.

Cryo-electron tomograms are enhanced by using rotational symmetry to average sub-tomogram density, improving interpretability. This method overcomes noise and missing wedge effects in macromolecular complex analysis.

Keywords:
Cryo-electron tomographyLocked self-rotation functionMacromolecular complexesPoint group symmetryVirus structure

More Related Videos

Deep Learning-Based Segmentation of Cryo-Electron Tomograms
10:25

Deep Learning-Based Segmentation of Cryo-Electron Tomograms

Published on: November 11, 2022

10.8K
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.8K

Related Experiment Videos

Last Updated: Jan 29, 2026

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements
14:18

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements

Published on: February 28, 2016

11.9K
Deep Learning-Based Segmentation of Cryo-Electron Tomograms
10:25

Deep Learning-Based Segmentation of Cryo-Electron Tomograms

Published on: November 11, 2022

10.8K
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.8K

Area of Science:

  • Structural biology
  • Biophysics
  • Microscopy

Background:

  • Cryo-electron tomography (cryo-ET) interpretation is challenging due to noise and the missing wedge effect.
  • Macromolecular complexes often exhibit inherent symmetries.

Purpose of the Study:

  • To develop and demonstrate a method for enhancing cryo-electron tomogram quality.
  • To leverage rotational symmetry for improved tomographic analysis of macromolecular complexes.

Main Methods:

  • Utilizing the rotational symmetry of samples to improve tomogram quality.
  • Determining symmetry axis orientation using a locked self-rotation function on sub-tomograms.
  • Averaging sub-tomogram density after determining symmetry for enhanced interpretability.

Main Results:

  • Demonstrated a procedure to enhance cryo-electron tomogram quality by exploiting rotational symmetry.
  • Successfully applied the method to sub-tomograms of the icosahedral bacteriophage phiX174.
  • Improved interpretability of tomographic data through density averaging.

Conclusions:

  • Rotational symmetry is a valuable property for enhancing cryo-electron tomogram analysis.
  • The locked self-rotation function and subsequent averaging provide a robust method for improving data quality.
  • This approach offers a significant advantage in interpreting noisy cryo-ET data of symmetric macromolecular complexes.