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Updated: Jan 29, 2026

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain
Published on: March 17, 2023
Transformation and regeneration of Lobelia erinus using Agrobacterium tumefaciens
1Department of Botany MC F0400, The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78713, USA e-mail: lloyd@uts.cc.utexas.edu Fax: +1-512-4713878, , , , , , US.
Abstract:
A transformation/regeneration system was developed for the common garden Lobelia (Lobelia erinus). Using an Agrobacterium-based protocol, over 40 transformants have been generated with four different binary vectors. The explant source was hypocotyl-root sections from axenically grown seedlings. Stable transformation was demonstrated by Southern hybridization analysis, β-glucuronidase staining, and transmission of the T-DNA to progeny. This extends the ever-widening range of transformable plant species to the Campanulaceae and will allow molecular studies of development and physiology in this easily cultured and popular garden plant.
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