In vitro propagation of Petasites hybridus (Asteraceae) from leaf and petiole explants and from inflorescence buds
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Updated: Jan 29, 2026

Asymbiotic Germination and Leaf Explant-Based Regeneration of the Endangered Medicinal Orchid Hemipilia cucullata from Mature Seeds
Published on: September 19, 2025
E Wildi1, W Schaffner1, K Berger Büter1
1Universität Basel, Institut für Pharmazie/ Pharmazeutische Biologie, Benkenstrasse 252, CH-4108 Witterswil, Switzerland, , , , , , CH.
Abstract:
In vitro shoot regeneration from sterile leaf and petiole explants and from in-situ-collected inflorescence buds of Petasites hybridus was achieved by a simple two-step protocol. Murashige and Skoog (MS) nutrient medium was supplemented with 17.6 μM benzyladenine (BA)+0.54 μM naphthaleneacetic acid (NAA) to induce shoots. After 5 weeks of culture, 40% of the petiole and 27% of the leaf explants produced shoots compared to 76% of the inflorescence buds. Single shoots were excised and subcultured on MS medium supplemented with various cytokinins (N6-(Δ2-isopentenyl)adenine, BA, kinetin and thidiazuron). A concentration of 8.8 μM kinetin+0.54 μM NAA performed best in terms of shoot multiplication rate, average shoot length and spontaneous root induction.
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