Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

5.2K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.2K
Migration00:53

Migration

8.9K
Migration is long-range, seasonal movement from one region or habitat to another. This common strategy, carried out by many different organisms around the world, is an adaptive response that typically corresponds to changes in an organism’s environment, like resource availability or climate. Migrations can involve huge groups of thousands of animals as well as single individuals traveling alone and can range from thousands of kilometers to just a few hundred meters.
8.9K
Cell Migration01:19

Cell Migration

6.6K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.6K
Cell Migration01:09

Cell Migration

18.7K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.7K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

5.1K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.1K
Cellular Differentiation00:57

Cellular Differentiation

5.3K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

panTB-HM: a multi-arm clinical trial of a pan-TB regimen targeting both host and microbe.

Trials·2026
Same author

Dietary intervention during pregnancy improves maternal diet index: The PEAPOD pilot trial.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2025
Same author

The adapted Caprini score as a proxy forpostoperative venous thromboembolismprophylaxis: a tertiary hospital experience.

South African journal of surgery. Suid-Afrikaanse tydskrif vir chirurgie·2025
Same author

High-Statistics Measurement of the Cosmic-Ray Electron Spectrum with H.E.S.S.

Physical review letters·2024
Same author

Bidirectional associations between IgE-mediated food allergy and atopic dermatitis.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2024
Same author

Combined therapeutic use of umbilical cord blood serum and amniotic membrane in diabetic wounds.

Biochimie·2024

Related Experiment Video

Updated: Jan 29, 2026

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins
11:01

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Published on: November 17, 2016

48.9K

Rapid quantification of cellular proliferation and migration using ImageJ.

C Venter1, C U Niesler1

  • 1Discipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville 3209, South Africa.

Biotechniques
|February 13, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed ImageJ protocols to quickly and accurately measure cell proliferation and migration. These methods improve upon manual analysis for cell counting and scratch assays, offering greater accuracy and efficiency.

Keywords:
ImageJadherent cell countsmigrationmyoblastproliferationscratch assay

More Related Videos

Outer-Boundary Assisted Segmentation and Quantification of Trabecular Bones by an Imagej Plugin
09:36

Outer-Boundary Assisted Segmentation and Quantification of Trabecular Bones by an Imagej Plugin

Published on: March 14, 2018

9.8K
Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
08:13

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Published on: December 27, 2016

19.4K

Related Experiment Videos

Last Updated: Jan 29, 2026

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins
11:01

Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Published on: November 17, 2016

48.9K
Outer-Boundary Assisted Segmentation and Quantification of Trabecular Bones by an Imagej Plugin
09:36

Outer-Boundary Assisted Segmentation and Quantification of Trabecular Bones by an Imagej Plugin

Published on: March 14, 2018

9.8K
Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs
08:13

Rapid Quantification of Mitogen-induced Blastogenesis in T Lymphocytes for Identifying Immunomodulatory Drugs

Published on: December 27, 2016

19.4K

Area of Science:

  • Cell biology
  • Biotechnology
  • Image analysis

Background:

  • Cellular proliferation and migration are fundamental biological processes critical for development, regeneration, and disease.
  • Existing methods for quantifying these processes, such as manual cell counts and scratch assays, are often time-consuming, labor-intensive, and require expensive reagents or equipment.
  • These limitations hinder high-throughput analysis and can introduce subjectivity.

Purpose of the Study:

  • To develop and optimize ImageJ-based protocols for the rapid and accurate quantification of adherent cell number and wound area.
  • To provide a cost-effective and accessible alternative to traditional manual methods for analyzing cell proliferation and migration.
  • To enhance the throughput and objectivity of cell-based assays.

Main Methods:

  • Utilized ImageJ, an open-source image processing software, to develop optimized quantification protocols.
  • Developed specific ImageJ procedures for automated cell counting to assess proliferation.
  • Adapted ImageJ for accurate measurement of wound closure area in scratch assays to evaluate migration.

Main Results:

  • The developed ImageJ protocols enable rapid quantification of both cell number and wound area.
  • These automated methods significantly improve accuracy compared to subjective manual identification and counting.
  • The protocols are adaptable and maintain high accuracy for adherent cell quantification.

Conclusions:

  • Optimized ImageJ protocols offer a rapid, accurate, and cost-effective solution for quantifying cellular proliferation and migration.
  • These ImageJ-based methods overcome the limitations of manual analysis, enabling higher throughput and more reliable results.
  • The developed protocols are valuable tools for researchers studying cell behavior in various biological contexts.