Phenotypic and molecular dissection of grain quality using the USDA rice mini-core collection

Grain quality is a major breeding objective and paramount to food production. This study was aimed to phenotypically and molecularly dissect the rice grain quality, especially amylose content (AC), grain protein content (GPC) and alkali spreading value (ASV), using the USDA rice mini-core collection representing the world-wide rice germplasm lines. Grain chemical analysis combined with genome-wide association study (GWAS) was used for the study. A wide genetic variation was observed for these grain quality traits in the mini-core collection. Germplasm lines unique in AC, GPC and ASV and desirable for grain quality improvement were identified. The genetic diversity of the collection was re-analyzed using new SNPs, thus providing a more precise genotypic information about the collection. Furthermore, ten loci significantly associated with these grain quality traits were identified through GWAS using 22947 high-quality SNPs. These results, therefore, provide knowledge, resources and molecular tools for efficient rice grain quality improvement.