Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structural Protein Function01:56

Structural Protein Function

29.9K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
29.9K
Structural Protein Function01:56

Structural Protein Function

3.3K
3.3K
Fruit Development, Structure, and Function01:58

Fruit Development, Structure, and Function

25.2K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.2K
Olfactory Receptors: Location and Structure01:03

Olfactory Receptors: Location and Structure

11.7K
The process of olfaction, also known as the sense of smell, is a sophisticated chemical response system. The specialized sensory neurons that facilitate this process, known as olfactory receptor neurons, are situated in an upper segment of the nasal cavity, known as the olfactory epithelium. Olfactory sensory neurons are bipolar, with their dendrites extending from the epithelium's apex into the mucus that lines the nasal cavity. Airborne molecules, when inhaled, traverse the olfactory...
11.7K
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

14.4K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
14.4K
Structure and Function of Erythrocytes01:29

Structure and Function of Erythrocytes

5.8K
There are between 4.2 and 6 million erythrocytes, also known as red blood cells, in every microliter of blood. These cells are small, flattened biconcave discs with centers that are depressed.
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
5.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology.

Journal of cell science·2025
Same author

Dual regulation of IP<sub>3</sub> receptors by IP<sub>3</sub> and PIP<sub>2</sub> controls the transition from local to global Ca<sup>2+</sup> signals.

Molecular cell·2024
Same author

KRAP regulates mitochondrial Ca2+ uptake by licensing IP3 receptor activity and stabilizing ER-mitochondrial junctions.

Journal of cell science·2024
Same author

Regulation of store-operated Ca<sup>2+</sup> entry by IP<sub>3</sub> receptors independent of their ability to release Ca<sup>2</sup>.

eLife·2023
Same author

What about nitrogen? Using nitrogen as a carrier gas during the analysis of petroleum biomarkers by gas chromatography mass spectrometry.

Journal of chromatography. A·2023
Same author

Dissociation of inositol 1,4,5-trisphosphate from IP<sub>3</sub> receptors contributes to termination of Ca<sup>2+</sup> puffs.

The Journal of biological chemistry·2023
Same journal

Evolutionary and Biochemical Perspectives on the Incorporation and Utilization of Selenocysteine.

Cold Spring Harbor perspectives in biology·2026
Same journal

The Mitochondrial Calcium Uniporter: From Parts to Signaling Networks.

Cold Spring Harbor perspectives in biology·2026
Same journal

Growth Control and Beyond: Functional Diversity and Regulation of the Hippo Pathway in the Nervous System.

Cold Spring Harbor perspectives in biology·2026
Same journal

Structural Studies of Core Hippo Pathway Components.

Cold Spring Harbor perspectives in biology·2026
Same journal

The Hippo Pathway in Intestinal Regeneration, Fetal Reprogramming, and Tumorigenesis.

Cold Spring Harbor perspectives in biology·2026
Same journal

A Synergy between Genetics and Biochemistry Unravels the Molecular Architecture of the Hippo Signaling Pathway.

Cold Spring Harbor perspectives in biology·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
11:31

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Published on: November 30, 2018

7.9K

Structure and Function of IP3 Receptors.

David L Prole1, Colin W Taylor1

  • 1Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, United Kingdom.

Cold Spring Harbor Perspectives in Biology
|February 13, 2019
PubMed
Summary
This summary is machine-generated.

Inositol 1,4,5-trisphosphate receptors (IP3Rs) release calcium (Ca2+) from the endoplasmic reticulum, initiating cellular calcium signals. Specific IP3R clusters at ER-plasma membrane junctions regulate calcium entry, crucial for cellular communication.

More Related Videos

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.8K
A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Published on: June 8, 2022

3.9K

Related Experiment Videos

Last Updated: Jan 29, 2026

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
11:31

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Published on: November 30, 2018

7.9K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.8K
A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Published on: June 8, 2022

3.9K

Area of Science:

  • Cellular Biology
  • Molecular Physiology
  • Calcium Signaling

Background:

  • Inositol 1,4,5-trisphosphate receptors (IP3Rs) mediate calcium (Ca2+) release from the endoplasmic reticulum (ER).
  • IP3Rs are crucial for initiating store-operated Ca2+ entry (SOCE) and propagating Ca2+ signals within animal cells.
  • The activation mechanism of IP3Rs involves IP3 binding followed by Ca2+ triggering.

Purpose of the Study:

  • To elucidate the structural basis of IP3R activation.
  • To understand the role of Ca2+-induced Ca2+ release in IP3R signaling.
  • To investigate the regulation of SOCE by specific IP3R clusters.

Main Methods:

  • Investigated IP3R subtypes and their interaction with IP3 and Ca2+.
  • Analyzed Ca2+ puffs as fundamental units of IP3-evoked Ca2+ signals.
  • Examined the localization of IP3R clusters at ER-plasma membrane junctions.

Main Results:

  • IP3 binding primes IP3Rs for Ca2+ binding, leading to channel opening.
  • Ca2+ puffs, arising from small IP3R clusters, are basic building blocks of Ca2+ signals.
  • Specific IP3R clusters at ER-plasma membrane junctions are 'licensed' to respond and may regulate SOCE.

Conclusions:

  • Understanding IP3R structure is key to deciphering Ca2+ signal generation.
  • Ca2+ puffs represent elementary regenerative Ca2+ events mediated by IP3Rs.
  • The strategic localization of IP3Rs near ER-plasma membrane junctions suggests a role in selectively controlling SOCE.