Related Experiment Video
Updated: Jan 29, 2026
09:58
Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
Published on: May 2, 2017
8.1K
EBV miR-BART10-3p Promotes Cell Proliferation and Migration by Targeting DKK1
Kyoungmi Min1, Suk Kyeong Lee1
1Department of Biomedicine & Health Sciences, Department of Medical Lifescience, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
International Journal of Biological Sciences
|February 13, 2019
Summary
Epstein-Barr virus (EBV)-associated gastric cancer involves BART miRNAs. miR-BART10-3p targets DKK1, influencing tumor progression by regulating cell proliferation and migration.
Area of Science:
- Molecular Biology
- Oncology
- Virology
Background:
- Epstein-Barr virus (EBV) infection is linked to epithelial cancers.
- BamHI A rightward transcript (BART) miRNAs are highly expressed in EBV-infected cancers.
- Limited knowledge exists regarding BART miRNA target genes in these cancers.
Purpose of the Study:
- To investigate the role of miR-BART10-3p in EBV-associated gastric carcinoma (EBVaGC).
- To determine if miR-BART10-3p directly targets DKK1.
- To explore the impact of the miR-BART10-3p/DKK1 axis on cancer cell behavior.
Main Methods:
- mRNA microarray analysis to identify differentially expressed genes.
- Luciferase reporter assay to confirm direct targeting of DKK1 3'-UTR by miR-BART10-3p.
- Transfection experiments using miR-BART10-3p mimics, inhibitors, siDKK1, and DKK1 overexpression vectors.
Main Results:
- DKK1 expression was significantly downregulated in EBVaGC cells.
- miR-BART10-3p directly targets and downregulates DKK1.
- miR-BART10-3p and siDKK1 promoted cell proliferation and migration.
- Inhibition of miR-BART10-3p or DKK1 overexpression suppressed proliferation and migration.
Conclusions:
- miR-BART10-3p plays a role in EBVaGC progression by targeting DKK1.
- The miR-BART10-3p/DKK1 pathway influences cancer cell proliferation and migration.
- This study identifies a novel regulatory mechanism in EBV-associated gastric cancer.
More Related Videos
Related Concept Videos
01:19Cell Migration
6.6K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.6K
01:09Cell Migration
18.7K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.7K
02:36Cells Coordinate Growth and Proliferation
5.1K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.1K
02:40The Eukaryotic Promoter Region
18.8K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
02:23Abnormal Proliferation
5.2K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.2K
01:35Cancer Cell Migration through Invadopodia
3.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.3K