Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Solvents01:12

Solvents

70.9K
A solvent is a substance, most often a liquid, that can dissolve other substances. Here, the substance being dissolved is called a solute. When a solvent and a solute combine, they form a solution - a homogenous mixture of both the solvent and the solute. Water is a universal biological solvent. Its polar structure allows it to dissolve many other polar compounds. The ability of water to dissolve is governed by a balance between water molecules binding to each other and binding to the solute.
A...
70.9K
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

1.1K
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
1.1K
Titration in Nonaqueous Solvents01:16

Titration in Nonaqueous Solvents

1.4K
Most acid-base titrations are performed in an aqueous medium. In aqueous titrations, water competes with weaker acids or bases for proton donation or acceptance, leading to ambiguous endpoints in the titration curve. Water also affects the partial ionization of weak acids or bases. For example, water accepts a proton from acetic acid to form hydronium and acetate ions. The hydronium ion formed is a stronger acid than acetic acid, and the acetate ion is a stronger base than water. As a result,...
1.4K
Organic Compounds03:02

Organic Compounds

57.2K
All living things are formed mostly of carbon compounds called organic compounds. The category of organic compounds includes both natural and synthetic compounds that contain carbon. Although a single, precise definition has yet to be identified by the chemistry community, most agree that a defining trait of organic molecules is the presence of carbon as the principal element, bonded to hydrogen and other carbon atoms. However, some carbon-containing compounds such as carbonates, cyanides, and...
57.2K
Overview of Advanced Functional Groups02:22

Overview of Advanced Functional Groups

29.8K

Functional groups are groups of atoms with specific chemical properties that occur within organic molecules and are sometimes denoted as “R”. Functional groups can “functionalize” a compound by enabling it to adopt different physical and chemical properties.
Types of Advanced Functional Groups
The table below summarizes some of the major functional groups in organic chemistry.
29.8K
Levels of Organization01:09

Levels of Organization

140.5K
Biological organization is the classification of biological structures, ranging from atoms at the bottom of the hierarchy to the Earth's biosphere. Each level of the hierarchy represents an increase in complexity that builds upon the previous level.
Molecules Are Composed of Atoms, and Biomolecules Are Assembled from Molecules:
The most basic levels include atoms, molecules, and biomolecules. Atoms, the smallest unit of ordinary matter, are composed of a nucleus and electrons. Molecules...
140.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transmissive-Detected Hyperspectral Imaging for Single-Vessel-Resolution Blood Oxygen Mapping.

BME frontiers·2025
Same author

Real-time self-supervised denoising for high-speed fluorescence neural imaging.

Nature communications·2025
Same author

1275-nm Photobiomodulation Alleviates Brain Drainage Impairment as a Promising Therapeutic Strategy for Aging-Related Neurological Decline.

Aging cell·2025
Same author

Diabetes Mellitus Impairs Blood-Brain Barrier Integrality and Microglial Reactivity.

Journal of biophotonics·2025
Same author

Strongly absorbing molecules make tissue transparent: a new insight for understanding tissue optical clearing.

Light, science & applications·2024
Same author

SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures.

Nature communications·2024
Same journal

Spatiotemporal control of myoblast identity drives muscle diversity in the <i>Drosophila</i> leg.

Science advances·2026
Same journal

Stellar feedback drives the baryon deficiency in low-mass galaxies.

Science advances·2026
Same journal

Antiferroelectric thin films embedded with ferroelectric switching loop for giant negative electrocaloric effect.

Science advances·2026
Same journal

Tetraphosphorylated phthalocyanine-based self-assembled monolayer stabilizes perovskite photovoltaics.

Science advances·2026
Same journal

Dual-mode analysis of ischemic stroke based on urine SERS spectra and carotid B-ultrasound.

Science advances·2026
Same journal

Remote homology and functional genetics unmask deeply preserved Scm3/HJURP orthologs in metazoans.

Science advances·2026
See all related articles

Related Experiment Video

Updated: Jan 29, 2026

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

3.5K

FDISCO: Advanced solvent-based clearing method for imaging whole organs.

Yisong Qi1,2, Tingting Yu1,2, Jianyi Xu1,2

  • 1Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Science Advances
|February 13, 2019
PubMed
Summary
This summary is machine-generated.

A new FDISCO method preserves fluorescence during optical clearing for deep biological imaging. This technique allows for long-term storage and detailed visualization of neuronal and vascular networks in whole organs.

More Related Videos

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
09:42

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

Published on: April 30, 2019

11.4K
Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts
07:10

Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts

Published on: May 16, 2021

5.3K

Related Experiment Videos

Last Updated: Jan 29, 2026

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
07:20

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales

Published on: May 10, 2022

3.5K
High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
09:42

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

Published on: April 30, 2019

11.4K
Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts
07:10

Refined CLARITY-Based Tissue Clearing for Three-Dimensional Fibroblast Organization in Healthy and Injured Mouse Hearts

Published on: May 16, 2021

5.3K

Area of Science:

  • Biomedical imaging
  • Optical clearing techniques
  • Histology

Background:

  • Optical clearing methods enable deep tissue visualization.
  • Existing methods like 3DISCO offer high clearing efficiency but quench fluorescence.
  • Loss of fluorescence limits the application of current clearing techniques in biological studies.

Purpose of the Study:

  • To develop an advanced optical clearing method that preserves endogenous and exogenous fluorescence.
  • To overcome the fluorescence quenching limitation of existing methods like 3DISCO.
  • To enable long-term storage and high-resolution imaging of cleared biological samples.

Main Methods:

  • Development of a novel FDISCO (Fluorescence-preserving DISCO) clearing protocol.
  • Evaluation of FDISCO's clearing efficiency and fluorescence preservation capabilities.
  • Application of FDISCO for high-resolution imaging of neuronal and vascular networks in whole organs.
  • Testing compatibility with viral labeling and weak fluorescence signals.

Main Results:

  • FDISCO effectively preserves fluorescence from various probes over months.
  • The method retains potent clearing capability for large biological samples.
  • High-resolution imaging and reconstruction of neuronal and vascular networks were achieved.
  • FDISCO enabled visualization of weakly fluorescent neurons in whole brains and is compatible with multiple viral labels.

Conclusions:

  • FDISCO is a superior alternative to 3DISCO for deep biological imaging, preserving fluorescence.
  • The method facilitates long-term storage of cleared samples while maintaining imaging quality.
  • FDISCO significantly advances three-dimensional mapping of whole organs for extensive biomedical research.