Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autophagy in cancer - functional plasticity, therapeutic paradox, and the road to precision modulation: a comprehensive review.

Molecular cancer·2026
Same author

Early-life exposures and risk of multiple gynecological diseases: evidence from a large community-based study of 272,706 women.

BMC women's health·2026
Same author

CD68<sup>+</sup> tumor-associated macrophages exhibit prognostic value in surgically resected small cell lung cancer: a retrospective cohort study of 614 patients.

Cancer immunology, immunotherapy : CII·2026
Same author

A novel high-sensitivity TaqMan qPCR assay reveals amdoparvovirus DNA in zoo-housed small mammals in southern China.

Veterinary research communications·2026
Same author

Corneal Stromal Microdots in Dry Eye Disease: Clinical Characterization and Associations With Corneal Nerve Parameters.

Translational vision science & technology·2026
Same author

Microalgae-Based Semiartificial Photosynthesis: Strategies, Applications, and Future Prospects.

Environmental science & technology·2026

Related Experiment Video

Updated: Jan 8, 2026

Non-Invasive Monitoring of Microvascular Oxygenation and Reactive Hyperemia using Hybrid, Near-Infrared Diffuse Optical Spectroscopy for Critical Care
14:28

Non-Invasive Monitoring of Microvascular Oxygenation and Reactive Hyperemia using Hybrid, Near-Infrared Diffuse Optical Spectroscopy for Critical Care

Published on: May 10, 2024

2.1K

Transmissive-Detected Hyperspectral Imaging for Single-Vessel-Resolution Blood Oxygen Mapping.

Shaojun Liu1,2, Qing Xia1, Yuwei Du1

  • 1MoE Key Laboratory for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Advanced Biomedical Imaging Facility, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, China.

BME Frontiers
|December 18, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces transmissive-detected hyperspectral imaging (TD-HSI) for improved deep-tissue blood oxygen mapping. TD-HSI offers higher precision and resolution than reflective methods, advancing noninvasive oxygen monitoring.

More Related Videos

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
11:35

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

Published on: December 8, 2010

16.9K
Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research
06:40

Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

Published on: June 8, 2022

2.3K

Related Experiment Videos

Last Updated: Jan 8, 2026

Non-Invasive Monitoring of Microvascular Oxygenation and Reactive Hyperemia using Hybrid, Near-Infrared Diffuse Optical Spectroscopy for Critical Care
14:28

Non-Invasive Monitoring of Microvascular Oxygenation and Reactive Hyperemia using Hybrid, Near-Infrared Diffuse Optical Spectroscopy for Critical Care

Published on: May 10, 2024

2.1K
Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
11:35

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

Published on: December 8, 2010

16.9K
Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research
06:40

Multispectral Optoacoustic Tomography for Functional Imaging in Vascular Research

Published on: June 8, 2022

2.3K

Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Physiology

Background:

  • Oxygen saturation (SO2) is vital for physiological monitoring.
  • Tissue scattering limits resolution and depth in reflective hyperspectral imaging (HSI).
  • Accurate deep-tissue oxygen mapping remains a challenge.

Purpose of the Study:

  • To develop and evaluate a transmissive-detected hyperspectral imaging (TD-HSI) strategy.
  • To overcome limitations of reflective-detected HSI (RD-HSI) for deep-tissue blood oxygen mapping.
  • To assess TD-HSI's potential for noninvasive, high-resolution in vivo oxygen monitoring.

Main Methods:

  • Monte Carlo simulations to compare RD-HSI and TD-HSI for deep-tissue SO2 measurement.
  • In vivo TD-HSI system implementation in mice.
  • Monitoring SO2 during induced hypoxia and tumor growth.

Main Results:

  • TD-HSI significantly extended the depth of accurate SO2 detection compared to RD-HSI.
  • TD-HSI demonstrated approximately 6-fold greater precision in detecting SO2 variations.
  • In vivo experiments achieved single-vessel resolution SO2 mapping in mice, surpassing RD-HSI capabilities.

Conclusions:

  • TD-HSI is a capable strategy for deep-tissue blood oxygen imaging.
  • TD-HSI offers substantial improvements over RD-HSI for noninvasive deep-tissue oxygen acquisition.
  • This technique provides a powerful tool for studying tissue oxygenation and microcirculation diseases.