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Migration inhibition of T lymphocytes from human peripheral blood by specific antigen and lymphokines
Abstract:
The inhibition of migration of human peripheral blood cells in the presence of PPD was studied. It was found that migration inhibition of peripheral blood mononuclear cells (MN) from Mantoux-positive donors was far greater than the migration inhibition of peripheral blood leucocytes (PBL). Moreover, MN cells and T lymphocytes showed larger and more uniform areas of migration. In contrast, the migration of B lymphocytes and monocytes was poor. Further analysis using purified subpopulations of MN cells showed that PPD inhibited the migration of T lymphocytes but not of B lymphocytes and monocytes. Corresponding to these findings, lymphokine-containing supernatants also inhibited the migration of purified T cells from Matoux-negative donors. It was concluded that the T lymphocyte was the predominant cell in the MN cell population, which migrated, and was subject to inhibition by PPD or lymphokines. These results imply that the movement of human T lymphocytes may be influenced by soluble factors from antigen-activated sensitized cells.
Insights
T lymphocytes are the primary cells inhibited by PPD and lymphokines, affecting human cell migration. This suggests soluble factors from activated cells influence T lymphocyte movement.
Area of Science:
- Immunology
- Cell Biology
Background:
- Cell migration is crucial for immune responses.
- Understanding factors influencing T lymphocyte migration is key to immune system function.
Purpose of the Study:
- To investigate the effect of purified protein derivative (PPD) on human peripheral blood cell migration.
- To identify which specific cell types within peripheral blood mononuclear cells are most affected by PPD and lymphokines.
Main Methods:
- Studied migration inhibition of human peripheral blood mononuclear cells (MN) and peripheral blood leucocytes (PBL) with PPD.
- Analyzed migration patterns of T lymphocytes, B lymphocytes, and monocytes.
- Examined the impact of lymphokine-containing supernatants on purified T cells.
Main Results:
- PPD significantly inhibited MN cell migration more than PBL.
- T lymphocytes exhibited greater and more uniform migration inhibition compared to B lymphocytes and monocytes.
- Purified T cells showed inhibition by PPD and lymphokines, while B cells and monocytes did not.
Conclusions:
- T lymphocytes are the predominant migratory cells in MN populations and are susceptible to PPD and lymphokine-induced migration inhibition.
- Soluble factors released by antigen-activated sensitized cells can influence the migration of human T lymphocytes.