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PpCBFs selectively regulate PpDAMs and contribute to the pear bud endodormancy process.

Jianzhao Li1,2,3, Xinhui Yan1,2,3, Qinsong Yang1,2,3

  • 1Department of Horticulture, Zhejiang University, Hangzhou, 310058, Zhejiang, People's Republic of China.

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Pear C-repeat binding factor 2 (PpCBF2) directly activates pear Dormancy Associated Module (DAM) genes, regulating leaf bud endodormancy. This study details the CBF-DAM signaling pathway

Keywords:
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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Endodormancy is crucial for temperate plant survival during winter.
  • Low temperatures regulate endodormancy, involving C-repeat binding factors (CBFs) and Dormancy Associated Module (DAM) genes in pear (Pyrus pyrifolia).

Purpose of the Study:

  • To investigate the detailed regulation of pear DAM genes by CBFs during endodormancy.
  • To elucidate the role of the CBF-DAM signaling pathway in 'Wonhwang' pear leaf bud endodormancy.

Main Methods:

  • Identification and expression analysis of CBF and DAM genes in 'Wonhwang' pear.
  • Biochemical assays (e.g., dual-luciferase assay) to determine gene promoter binding and transcriptional activation.
  • Analysis of ICE-CBF regulon components and their response to chilling conditions.

Main Results:

  • PpCBF2 and PpCBF4 bind to the PpDAM1 promoter and activate its expression.
  • PpCBF1 and PpCBF4 activate PpDAM3 expression.
  • PpCBF2 directly binds to PpCBF3 and PpCBF4 promoters, activating their transcription.
  • The PpCBF1-PpDAM2 regulon is key for low-temperature response in endodormancy, potentially modulated by PpICE3.

Conclusions:

  • PpCBF2 plays a direct role in regulating specific PpDAM genes during pear endodormancy.
  • The CBF-DAM signaling pathway is integral to pear bud endodormancy.
  • Findings offer insights into CBF gene functional redundancy and differentiation in plants.