Related Experiment Video
Updated: Jan 29, 2026

Screening Cotton Genotypes for Reniform Nematode Resistance
Published on: May 2, 2019
Genotype screening for proliferative embryogenesis and biolistic transformation of short-season soybean genotypes
1Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Bldg. 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 e-mail: simmondsdh@em.agr.ca Fax: +613-7596566, , , , , , CA.
Abstract:
Eighteen of 20 short-season soybean (Glycine max (L.) Merrill) genotypes (maturity group 0 and 00) screened for proliferative embryogenic capacity formed secondary globular embryos, at rates of 1-70% of cultured immature cotyledons. Five genotypes produced embryogenic cultures which were proliferative for at least 6 months. Proliferative embryogenic cultures of AC Colibri and X2650-7-2-3 were bombarded using a Bio-Rad PDS-1000/He particle gun. Co-bombardments with plasmid pairs pHygr (encoding a type IV aminoglycoside phosphotransferase;aphIV) and pRD300pat (encoding a phosphinothricin N-acetyltransferase;pat) or pRD300pat and pFF19G (β-glucuronidase;uidA or gus) resulted, respectively, in 12 hygromycin-selected lines with multiple insertions of aphIV and pat, and two l-phosphinothricin-selected lines plus three β-glucuronidase-positive lines recovered without selection. Although fertile plants were recovered from young proliferative cultures, transgenic plants, which were derived from cultures 12-14 months of age, were sterile.
Related Concept Videos
Seasoning of Wood
Achieving equilibrium moisture content is the goal of seasoning; this is the point where the wood's moisture content stabilizes to align with the moisture levels of the surrounding environment. Proper...
Proliferative Phase
Notably, the stratum basale, the basal layer of the endometrium, including the basal parts of the uterine glands, remains unaffected by menstruation. Stem cells in this layer undergo mitosis, regenerating the stratum functionalis and thickening the...
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Biological Clocks and Seasonal Responses
Transformers
The iron core has a substantial relative permeability. Therefore, the magnetic field lines generated due to the current in one winding are almost entirely confined within the core, such that the same magnetic flux permeates each turn of both...
Transformation

