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Updated: Jul 29, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Published on: November 28, 2019
Re: "Amniotic fluid from healthy term pregnancies does not harbor a detectable microbial community" (2018) 6:87,
M S Payne1, J A Keelan2,3, L F Stinson2
1Division of Obstetrics and Gynaecology, School of Medicine, University of Western Australia, 35 Stirling Highway, Crawley, Western Australia, Australia. matthew.payne@uwa.edu.au.
Abstract:
A recent publication by Lim et al. 2018 (Amniotic fluid from healthy term pregnancies does not harbor a detectable microbial community) strongly concluded that the microbiome of amniotic fluid primarily originates from reagent contamination. However, upon closer inspection of the methods used and data presented in this study, in particular the supplementary data, such conclusions do not appear to be supported by the results. We outline such methodological/data interpretation concerns and invite the authors to discuss these.
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