Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mismatch Repair01:36

Mismatch Repair

43.7K
Overview
43.7K
Mismatch Repair01:20

Mismatch Repair

6.5K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
6.5K
DNA Base Pairing02:27

DNA Base Pairing

33.1K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
33.1K
DNA Base Pairing02:27

DNA Base Pairing

32.2K
32.2K
Overview of DNA Repair02:25

Overview of DNA Repair

33.6K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
33.6K
Characteristics and Nomenclature of Copolymers01:24

Characteristics and Nomenclature of Copolymers

3.3K
Copolymers are the products obtained from the polymerization of multiple monomer species. So, in a polymer chain itself, there can be multiple repeating units that come from different monomers. The process of synthesizing a polymer from different monomer species is called copolymerization. When two monomers are involved, the polymer is known as a bipolymer. Polymers with three and four monomers are termed terpolymers and quaterpolymers, respectively. Figure 1 depicts the copolymerization of...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alternating copolymerization of l-lactide and ε-caprolactone <i>via</i> enantiomorphic site and chain-end synergistic control.

Chemical science·2026
Same author

Design and On-Orbit Validation of a Compact Wide-Swath Spaceborne SWIR Push-Broom Camera.

Sensors (Basel, Switzerland)·2026
Same author

Combined Target-Immobilized and Library-Immobilized SELEX for Selecting High-Affinity α-Amanitin Aptamers.

Toxins·2026
Same author

Long-Distance Free-Space Quantum Key Distribution with Continuous Variables.

Physical review letters·2026
Same author

Photochromism Mechanism of <i>N</i>,<i>C</i>-Chelating Organoboron Compounds.

Inorganic chemistry·2026
Same author

Large-aperture telescope system based on a secondary optical beam expander with tracking precision of 100 nanoradians.

Optics letters·2026

Related Experiment Video

Updated: Jan 29, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

14.3K

Fluorescent DNA Biosensor for Single-Base Mismatch Detection Assisted by Cationic Comb-Type Copolymer.

Jialun Han1, Jincai Wu2, Jie Du3

  • 1State Key Laboratory of Marine Resource Utilization in South China Sea, College of materials and chemical engineering, Hainan University, Haikou 570228, China. jialun_han@126.com.

Molecules (Basel, Switzerland)
|February 16, 2019
PubMed
Summary

This study presents a rapid, cost-effective DNA mismatch detection assay using graphene oxide and a special copolymer. The method efficiently identifies single nucleotide polymorphisms (SNPs) for genetic disease diagnosis.

Keywords:
SNP analysiscationic comb-type copolymerfluorescent DNA biosensorgraphene oxidesingle-base mismatch detection

More Related Videos

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

34.6K
Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
10:28

Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis

Published on: February 8, 2010

11.7K

Related Experiment Videos

Last Updated: Jan 29, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
17:16

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

14.3K
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

34.6K
Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis
10:28

Imaging Mismatch Repair and Cellular Responses to DNA Damage in Bacillus subtilis

Published on: February 8, 2010

11.7K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Accurate detection of DNA single base mismatches and point mutations is crucial for diagnosing genetic diseases and understanding single nucleotide polymorphisms (SNPs).
  • Homogeneous mutation assays offer advantages in speed and signal amplification for automated detection.

Purpose of the Study:

  • To develop a quick, cost-effective, and sensitive assay for SNP analysis.
  • To leverage graphene oxide's properties and a novel copolymer to enhance DNA hybridization and mismatch discrimination.

Main Methods:

  • Utilized a fluorescent single-labeled DNA probe with graphene oxide (GO) for its preferential binding to single-stranded DNA (ssDNA) and quenching effect.
  • Incorporated a cationic comb-type copolymer (CCC), poly(l-lysine)-graft-dextran (PLL-g-Dex), to accelerate DNA hybridization and strand-exchange kinetics.
  • Evaluated the assay's sensitivity and practicality through in vitro experiments, including cell culture on PLL-g-Dex.

Main Results:

  • The assay demonstrated efficient quenching by GO and preferential binding to ssDNA.
  • The PLL-g-Dex copolymer significantly enhanced the kinetic discrimination between perfectly matched and mismatched DNA.
  • In vitro cell culture experiments confirmed the biocompatibility of PLL-g-Dex with RAW 264.7 cells, indicating practical applicability.

Conclusions:

  • The developed assay provides a highly sensitive and practical method for detecting DNA mismatches and SNPs.
  • The combination of GO and PLL-g-Dex offers a promising strategy for rapid genetic analysis and disease diagnostics.