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Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish

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Single cell immune profiling in transplantation research.

Lauren E Higdon¹, Steven Schaffert^2,3, Purvesh Khatri^2,3

¹Department of Medicine/Nephrology, Stanford University, Palo Alto, California.

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons

|February 16, 2019

Summary

Single-cell immune profiling technologies offer new insights into cell populations and gene expression. This review covers key analysis methods, including bioinformatics, for these complex datasets, especially for transplantation research.

Keywords:

basic (laboratory) research/science flow cytometry genetics genomics immunobiology informatics molecular biology translational research/science

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Single-cell Profiling of Developing and Mature Retinal Neurons

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Published on: April 19, 2012

Area of Science:

Immunology
Bioinformatics
Genomics

Background:

Single-cell profiling technologies are advancing the study of immune cell heterogeneity and gene expression.
These advanced techniques generate complex datasets requiring specialized bioinformatics expertise for accurate interpretation.

Purpose of the Study:

To review single-cell immune profiling technologies for gene and protein expression.
To discuss critical data analysis considerations for these technologies.
To provide examples for applying these methods in transplantation research.

Main Methods:

Cytometry by time-of-flight
RNA sequencing
Antigen receptor sequencing
Bioinformatic analysis pipelines

Main Results:

Discussion of quality control, quantification, and high-dimensional analysis methods.
Exploration of immune repertoire analysis techniques.
Guidance on preparing robust analysis pipelines for single-cell data.

Conclusions:

Effective data analysis is crucial for high-parameter single-cell studies.
These technologies and analysis strategies can yield significant insights in transplantation research.