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First Detection of Grapevine leafroll-associated virus 4 in Chilean Grapevines
P F Escobar1, N Fiore2, P D T Valenzuela3
1Fundación Ciencia para la Vida and MIFAB, Zañartu 1482, Santiago, Chile.
Plant Disease
|February 17, 2019
Summary
Grapevine leafroll-associated virus 4 (GLRaV-4) has been detected for the first time in Chilean grapevines. This finding is crucial for understanding the spread of this economically significant grapevine virus in the region.
Area of Science:
- Plant Virology
- Molecular Diagnostics
- Agricultural Science
Background:
- Grapevine leafroll disease is a major economic threat to vineyards worldwide.
- Multiple Grapevine leafroll-associated viruses (GLRaVs) cause this disease, with GLRaV-4 previously identified in California.
- Understanding the geographical distribution of GLRaVs is vital for disease management.
Purpose of the Study:
- To investigate the presence of Grapevine leafroll-associated virus 4 (GLRaV-4) in Chilean grapevines.
- To screen grapevine samples for GLRaV-4 using molecular methods.
- To establish the initial report of GLRaV-4 in Chile.
Main Methods:
- Dormant cane samples from 12 grapevine cultivars across Chile were collected.
- Reverse transcription-polymerase chain reaction (RT-PCR) was employed for virus detection.
- Two distinct primer pairs targeting the HSP70h and coat protein genes of GLRaV-4 were utilized.
Main Results:
- GLRaV-4 was detected in two Thompson Seedless cv. samples from Chile's III and VI regions.
- Sequencing confirmed high nucleotide identity (98.4-99.6%) with known GLRaV-4 isolates from California and Israel.
- This marks the first documented occurrence of GLRaV-4 in Chile.
Conclusions:
- GLRaV-4 is present in Chilean grapevines, expanding its known geographical distribution.
- The molecular detection methods used were effective for identifying GLRaV-4.
- Further research is needed to assess the impact and prevalence of GLRaV-4 in Chilean viticulture.
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