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Updated: Jan 29, 2026

A Method to Study α-Synuclein Toxicity and Aggregation Using a Humanized Yeast Model
Published on: November 25, 2022
Monitoring α-Synuclein Proteotoxicity in Drosophila Models
Aron Szabo1, George K Tofaris2
1Institute of Genetics, Biological Research Centre of the Hungarian Academy of Sciences, Szeged, Hungary.
Abstract:
Parkinson's disease is the second most common neurodegenerative disease without cure. It is characterized by α-synuclein accumulation and aggregation in dopaminergic and other types of neurons. Because α-synuclein accumulation leads to a toxic gain of function, its ectopic expression in Drosophila has been a useful in vivo model for testing modifiers of its toxicity. This chapter describes four assays: the rapid iterative negative geotaxis, rough eye phenotype, quantification of dopaminergic neuronal loss, and measurements of circadian effects.
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