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Published on: August 22, 2007
A guide to selecting high-performing antibodies for Netrin-1 (UniProt ID: O95631) for use in western blot and
Riham Ayoubi1, Sara González Bolívar1, Richard A Kahn1
1Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Canada.
Abstract:
Netrin-1 is a secreted protein that regulates cell migration and survival, controlling axonal guidance during development, morphogenesis, apoptosis, angiogenesis, inflammation and cancer progression. Here we have characterized fourteen Netrin-1 commercial antibodies for western blot and immunoprecipitation using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While the use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.
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