A promising voltammetric biosensor based on glutamate dehydrogenase/Fe₃O₄/graphene/chitosan nanobiocomposite for...

A novel NH₄⁺ voltammetric electrochemical biosensor was constructed by immobilizing glutamate dehydrogenase (GLDH)/Fe₃O₄/graphene (GR)/chitosan (CS) nanobiocomposite onto a glassy carbon electrode (GCE). On the GLDH/Fe₃O₄/GR/CS/GCE, GLDH catalyzed the reversible reaction, i.e., the reductive amination of α-ketoglutaric acid and the oxidative deamination of L-glutamate. The electrons produced in the enzymatic reactions were transferred to the surface of the electrode via the [Fe(CN)₆]^3-/4- couple, which helped for the amplification of the electrochemical signal. The electrochemical detection of NH₄⁺ was based on the fact that the enhanced response current was proportional to the NH₄⁺ concentration. Owing to the combination of the advantages of the synergistic effects of Fe₃O₄ nanospheres, GR and CS, a promising platform for NH₄⁺ sensing was provided. Under optimum conditions, the introduced biosensor had a linear range of 0.4-2.0 μM for NH₄⁺ with the detection and quantification limits of 0.08 and 0.27 μM, respectively. Moreover, the biosensor exhibited good sensitivity and excellent reproducibility. It could retain 91.8% of its original response after two weeks of storage at 4 °C, suggesting satisfactory stability. Additionally, the proposed biosensor was successfully applied to detect NH₄⁺ levels in PM_2.5 samples, indicating its feasibility for application in NH₄⁺monitoring in the environmental fields.