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Updated: Jan 29, 2026

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Published on: March 22, 2020
Ligand-Induced Structural Changes of Thiolate-Capped Gold Nanoclusters Observed with Resistive-Pulse Nanopore Sensing
Bobby D Cox1, Patrick H Woodworth1, Peter D Wilkerson1
1Department of Physics , Virginia Commonwealth University , Richmond , Virginia 23284 , United States.
Abstract:
Nanopore-based resistive pulse sensing with biological nanopores has traditionally been applied to biopolymer analysis, but more recently, interest has grown in applying the technique to characterizing water-soluble metallic clusters. This paper reports on the use of α-hemolysin (αHL) for detecting a variety of thiolate-capped gold nanoclusters. The ligands studied here are p-mercaptobenzoic acid ( p-MBA), tiopronin (TP), and thiolated PEG7 (S-PEG7). Individual clusters trapped in the cis-side of an αHL pore for extended periods (>10 s) exhibit fluctuations between numerous substates. We compare these current steps between the three different ligands and find that they scale with the mass of the corresponding ligand, which suggests that nanopore sensing could be used to characterize intraparticle surface modifications.
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