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Updated: Jul 21, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Engineering Biological Nanopore Approaches toward Protein Sequencing.

Xiaojun Wei^1,2, Tadas Penkauskas^3,4, Joseph E Reiner⁵

¹Biomedical Engineering Program, University of South Carolina, Columbia, South Carolina 29208, United States.

|July 25, 2023

Summary

This summary is machine-generated.

Nanopore technology shows promise for single-molecule protein sequencing, overcoming current limitations in protein identification. Further engineering and interdisciplinary collaboration are key to realizing its potential for analyzing individual amino acids and proteins.

Keywords:

amino acid engineering instrumentation machine learning molecular simulation nanopore peptide protein sequencing

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Last Updated: Jul 21, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

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Published on: June 3, 2019

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Published on: December 14, 2017

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

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Published on: March 15, 2019

Area of Science:

Biotechnology
Nanotechnology
Biophysics

Background:

Current protein studies are limited by inadequate single-molecule identification and quantification methods.
Nanopore systems, successful in genome sequencing, are now being explored for protein analysis.

Purpose of the Study:

To review recent advancements in biological nanopore sensors for protein sequencing.
To provide engineering-focused recommendations for developing nanopore-based protein sequencing.

Main Methods:

Review of recent advances in biological nanopore sensors.
Analysis of device and algorithm development for protein translocation and identification.
Utilizing molecular simulations to understand underlying mechanisms.

Main Results:

Nanopore systems demonstrate potential for protein identification, detection, and analysis at the single-molecule level.
Progress has been made in identifying individual amino acids and controlling peptide/protein translocation.

Conclusions:

Nanopore-based protein sequencing offers realistic prospects for novel analytical capabilities.
Advancement requires interdisciplinary collaboration, including chemical conjugation, protein engineering, simulation, machine learning, and device fabrication.