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Updated: May 6, 2026

A Technique for Stabilizing Membrane Proteins in Nanodiscs

A Technique for Stabilizing Membrane Proteins in Nanodiscs

Published on: April 30, 2026

DNA-Lipid Nanodiscs with a Polyethylene Glycol Interface.

Soumya Chandrasekhar^1,2, Christopher Maffeo^3,4, Sanjai Karanth¹

¹Department of Physics, Kent State University, Kent, Ohio 44242, United States.

Journal of the American Chemical Society

|May 4, 2026

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Researchers developed novel DNA-lipid nanodiscs (DLNs) for studying membrane proteins. These nanodiscs use DNA scaffolds and PEG linkers to create stable lipid bilayers, enabling protein incorporation and analysis.

Area of Science:

Biochemistry
Nanotechnology
Molecular Biology

Background:

Nanodiscs are essential tools for membrane protein research.
Existing nanodiscs face challenges in stability and precise structural control.

Purpose of the Study:

To introduce DNA-lipid nanodiscs (DLNs) as a new platform for membrane studies.
To demonstrate the ability of DLNs to incorporate membrane proteins.

Main Methods:

Synthesizing PEG-modified oligonucleotides into functionalized DNA minicircles.
Forming lipid bilayers within the DNA scaffold via detergent removal.
Incorporating a biotinylated transmembrane protein domain into DLNs.
Utilizing streptavidin-conjugated quantum dots for detection.

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Main Results:

Successfully created DNA-lipid nanodiscs with a defined diameter.
Simulations confirmed PEG's role in mediating hydrophobic mismatch.
Demonstrated successful incorporation of a membrane protein domain into DLNs.
Verified protein incorporation using quantum dot labeling.

Conclusions:

DNA-lipid nanodiscs offer a precise and modular scaffold for membrane protein research.
DLNs provide a versatile tool for studying lipid bilayers and membrane protein function.
This technology has broad applicability in nanobiotechnology and membrane biophysics.