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Related Experiment Video

Updated: Jun 3, 2026

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
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Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

Published on: June 3, 2011

Intracellular neuronal recordings across DNA tiles.

Shulan Xiao1, Sang Hoon Um1, Meng Xu2,3

  • 1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.

Nature Nanotechnology
|June 1, 2026
PubMed
Summary

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This summary is machine-generated.

DNA origami tiles enable stable ion transport and intracellular access in neurons, overcoming challenges in biohybrid electronics and neural interfaces. This technique facilitates drug delivery and voltage measurements without disrupting cell function.

Area of Science:

  • Nanotechnology
  • Neuroscience
  • Bioelectronics

Background:

  • Artificial ion channels are crucial for biohybrid electronics and neural interfaces.
  • Stable intracellular access via synthetic channels remains a significant challenge.
  • Current methods struggle with accessing thin neuronal structures.

Purpose of the Study:

  • To demonstrate stable transmembrane ionic flow using DNA origami tiles in live neurons.
  • To establish a novel method for intracellular access and drug delivery.
  • To enable intracellular voltage measurements without membrane disruption.

Main Methods:

  • Utilized DNA origami tiles (0.8 nm diameter) anchored into neuronal membranes.
  • Employed patch-clamp techniques, including two-photon-targeted variants.

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Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats
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Related Experiment Videos

Last Updated: Jun 3, 2026

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
10:29

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

Published on: June 3, 2011

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
12:51

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Published on: November 29, 2012

Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats
08:41

Juxtacellular Monitoring and Localization of Single Neurons within Sub-cortical Brain Structures of Alert, Head-restrained Rats

Published on: April 27, 2015

  • Performed molecular dynamics simulations to support experimental findings.

    • Main Results:

    • Achieved highly stable ion transport (~2 nS) with channel-like stochasticity.
    • Demonstrated successful intracellular drug delivery without compromising neuronal physiology.
    • Obtained repeatable intracellular and quasi-intracellular voltage measurements, even in thin dendrites (~1 µm).

    Conclusions:

    • DNA origami tiles provide a stable and effective means for transmembrane access in neurons.
    • This 'outside looking in' approach bypasses the need for membrane break-in, enabling new methods for probing neuronal dynamics.
    • The technology holds promise for advancing neural interface technologies and intracellular drug delivery systems.