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Updated: Jan 29, 2026

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
Published on: December 22, 2020
Analysis of monodisperse, sequence-defined, and POSS-functionalized polyester copolymers by MALDI tandem mass
Jialin Mao1, Wei Zhang2, Stephen Zd Cheng2
11 Department of Chemistry, The University of Akron, Akron, OH, USA.
Abstract:
Monodisperse, sequence-defined polymers can be potentially used for digital data storage. This study reports the sequence analysis and differentiation of monodisperse polyester copolymers carrying side chains functionalized in a specific order by polyhedral oligomeric silsesquioxane (POSS) nanoparticles. Steglich esterification and succinic anhydride ring-opening chemistries were utilized iteratively to synthesize the intended sequences, which were characterized by matrix-assisted laser desorption ionization tandem mass spectrometry (MALDI-MS2). Isomeric oligomers were readily distinguished based on their different fragmentation patterns. The sequences embedded in the oligomers were decrypted by their specific backbone dissociation pathways. The robustness of using MALDI-MS2 as a sequencing method for monodisperse synthetic macromolecules was assessed and validated by the characterization of longer oligomers.
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