Related Experiment Video
Updated: Jan 29, 2026
11:40
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
3.0K
Recent advances in proximity-based labeling methods for interactome mapping
1Department of Cellular and Molecular Medicine and Ottawa Institute of Systems Biology, Faculty of Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
F1000Research
|February 19, 2019
Summary
Proximity labeling techniques like BioID and APEX map protein interactions by labeling nearby proteins. Recent advances enable RNA and DNA proximity labeling for broader biological insights.
Area of Science:
- Biochemistry
- Molecular Biology
- Proteomics
Background:
- Proximity-based labeling complements traditional affinity purification for mapping protein-protein interactions.
- Enzyme tag and delivery method optimization enhance temporal and spatial resolution.
- Successful application in small-scale (single complex) and large-scale (network) mapping initiatives.
Purpose of the Study:
- To review the advancements and applications of proximity-based labeling techniques.
- To highlight the utility of these methods in mapping dynamic protein interactomes.
- To introduce novel RNA- and DNA-centric proximity labeling assays.
Main Methods:
- Utilizing enzyme tags (e.g., BioID, APEX) for proximity-dependent biotinylation.
- Employing quantitative proteomic approaches to identify labeled proteins.
- Developing RNA-centric and DNA-centric labeling strategies.
Main Results:
- Proximity labeling provides snapshots of stable and transient protein interactions.
- Enhanced resolution facilitates the study of dynamic interactomes.
- Expansion to RNA and DNA targets broadens the scope of interaction mapping.
Conclusions:
- Proximity labeling is a versatile tool for interactome mapping.
- Recent innovations extend its application beyond protein-centric studies.
- These techniques are crucial for understanding complex biological networks.
Related Concept Videos
00:56Extraction: Advanced Methods
1.1K
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
1.1K
02:22Overview of Advanced Functional Groups
29.7K
Functional groups are groups of atoms with specific chemical properties that occur within organic molecules and are sometimes denoted as “R”. Functional groups can “functionalize” a compound by enabling it to adopt different physical and chemical properties.
Types of Advanced Functional Groups
The table below summarizes some of the major functional groups in organic chemistry.
29.7K
01:22Factors Influencing Attraction I: Proximity
264
Proximity plays a fundamental role in shaping interpersonal attraction by increasing opportunities for interaction and fostering familiarity. Research consistently demonstrates that individuals are more likely to form social bonds with those who are physically closer to them, whether in residential settings, workplaces, or educational institutions. This effect is largely driven by the increased frequency of encounters, which facilitates the development of friendships and romantic...
264
01:42Microtubule Associated Proteins (MAPs)
5.9K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
5.9K
01:29Plotting of Topographic Maps
510
Topographic maps represent the Earth's surface features using contour lines, which connect points of equal elevation to create a two-dimensional representation of three-dimensional terrain. Creating a topographic map requires a systematic approach.Begin by plotting a scaled grid and marking intersections corresponding to the survey's elevation data points. Assign elevation values at these intersections to build the base map. Next, determine contour levels using a consistent contour interval,...
510
01:29Coordinates and Map Projections
605
Coordinates and map projections are essential tools in accurately representing the Earth's surface for various applications, ranging from navigation to spatial analysis. The latitude and longitude coordinate system is a universally recognized framework for defining locations. Latitude specifies the distance of a point north or south of the equator, measured in degrees from 0° at the equator to 90° at the poles. Longitude indicates a location's position east or west of the prime meridian,...
605