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We developed a multi-modal sensorless adaptive optics (SAO) imaging system for non-invasive, single-cell visualization in mouse retinas. This system enables detailed observation of microglia dynamics and cellular structures within the inner retina.

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Area of Science:

  • Ophthalmology
  • Neuroscience
  • Biomedical Engineering

Background:

  • Small animal models are crucial for vision research, offering transgenic strains for disease modeling and biomarker expression.
  • Adaptive optics (AO) allows non-invasive single-cell imaging in vivo but often involves complex systems.
  • Sensorless AO (SAO) offers a simpler approach for aberration correction and depth-resolved imaging.

Purpose of the Study:

  • To present a multi-modal sensorless AO (SAO) en face retina imaging system.
  • To demonstrate a compact, lens-based design with a flexible field of view (FOV).
  • To validate the system's capability for in vivo cellular imaging in mice.

Main Methods:

  • Developed a multi-modal SAO system integrating optical coherence tomography (OCT), OCT-angiography, confocal scanning laser ophthalmoscopy (SLO), and fluorescence detection.
  • Designed a compact, lens-based system with a 50-degree FOV, adjustable to the region of interest.
  • Employed SAO for aberration correction with chosen imaging modalities.

Main Results:

  • Successfully imaged microglia (Cx3cr1-GFP) dynamics over an hour using SAO SLO, observing clear changes in microglial branches.
  • Demonstrated volumetric cellular imaging of microglia throughout the inner retina.
  • Validated system performance on wild-type and transgenic mice.

Conclusions:

  • The presented multi-modal SAO system provides a low-complexity, effective solution for non-invasive, high-resolution retinal imaging in small animals.
  • The system facilitates dynamic cellular imaging and volumetric analysis, advancing vision research capabilities.
  • This technology supports the study of cellular processes and disease mechanisms in vivo.