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Updated: Jan 29, 2026

Repeatable Stair-step Assay to Access the Allelopathic Potential of Weedy Rice Oryza sativa ssp.
Published on: January 28, 2020
RNA Extraction and Preparation in Rice (Oryza sativa)
Cui-Cui Yin1, Biao Ma1, Wei Wang1
1Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Abstract:
There has been a surge of interest in the molecular biology of RNA. We describe a simple, fast, and convenient protocol for isolating total RNA from rice (Oryza sativa) seedlings and young tissues/organs. RNA obtained using this method can be used for reverse transcription-polymerase chain reaction (RT-PCR), quantitative RT-PCR (qRT-PCR), northern blot analysis, RNA-seq, microarray analysis, cDNA library construction, etc. Technical parameters critical to the success of the extraction are presented. © 2016 by John Wiley & Sons, Inc.
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