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Updated: Jan 29, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
Published on: May 7, 2009
Microinjection of oocytes and embryos with synthetic mRNA encoding molecular probes
George von Dassow1, Jenna Valley1, Kara Robbins1
1Oregon Institute of Marine Biology, University of Oregon, Charleston, OR, United States.
Abstract:
We describe methods and techniques for introduction of molecular probes in the form of synthetic mRNA by rapid repetitive microinjection into oocytes or early embryos of echinoderms and various invertebrates. Construct assembly is followed by standard kit-based in vitro mRNA synthesis, with slight modifications to optimize expression and clean-up. Variations of a basic microinjection procedures are detailed for echinoderms: starfish oocytes (Patiria miniata or other species), purple urchin (Strongylocentrotus purpuratus) and sand dollar (Dendraster excentricus) zygotes, with notes included for other invertebrate eggs and embryos as well.
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