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Cidaroids, clypeasteroids, and spatangoids: Procurement, culture, and basic methods.
Taku Hibino1, Takuya Minokawa2, Atsuko Yamazaki3
1Faculty of Education, Saitama University, Sakura-ku, Saitama City, Japan.
Methods in Cell Biology
|February 20, 2019
Summary
This guide details practical methods for using non-camarodont echinoids, such as sand dollars and heart urchins, in biological research. It covers collection, maintenance, and experimental techniques for these marine invertebrates.
Area of Science:
- Marine Biology
- Developmental Biology
- Echinoderm Research
Background:
- Non-camarodont echinoids, including cidaroids, clypeasteroids (sand dollars), and spatangoids (heart urchins), are valuable models for biological studies.
- Established protocols for the use of these species in research are essential for advancing scientific understanding.
Purpose of the Study:
- To provide practical methods and key insights for utilizing specific non-camarodont echinoids in biological experiments.
- To serve as a comprehensive resource for researchers working with Japanese echinoid species.
Main Methods:
- Collection and laboratory maintenance of adult echinoids.
- Embryonic culture techniques for developmental studies.
- Experimental procedures including micromanipulation, whole mount in situ hybridization, and immunological assays.
Main Results:
- Detailed protocols are presented for handling and culturing six Japanese echinoid species: Astriclypeus manni, Clypeaster japonicus, Echinocardium cordatum, Peronella japonica, Prionocidaris baculosa, and Scaphechinus mirabilis.
- The chapter outlines effective methods for various experimental manipulations on these echinoids.
Conclusions:
- This chapter offers a practical framework for researchers to effectively use non-camarodont echinoids in diverse biological investigations.
- The provided methods facilitate research in developmental biology, genetics, and immunology using these marine invertebrates.
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