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[Distribution of peripheral blood lymphocyte subsets in 826 healthy children aged 0-6 years]

Wen-Li Zhan1, Xiao-Han Yang, Hao Guo

  • 1Medical Genetics Center, Guangdong Women and Children's Hospital, Guangzhou 511442, China. luo-my@163.com.

Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics
|February 21, 2019
PubMed

Insights

Peripheral blood lymphocyte subset distribution varies significantly in children aged 0-6 years by both age and sex. Establishing age- and sex-specific reference values for lymphocyte subsets is crucial for accurate health assessments.

Area of Science:

  • Immunology
  • Pediatrics
  • Hematology

Background:

  • Understanding the normal range of immune cell populations in children is essential for diagnosing and managing various pediatric conditions.
  • Lymphocyte subset distribution is known to change dynamically during early childhood development.

Purpose of the Study:

  • To investigate the age- and sex-specific distribution patterns of peripheral blood lymphocyte subsets in healthy children aged 0-6 years.
  • To provide data that can inform the establishment of age- and sex-specific reference values.

Main Methods:

  • A cohort of 826 healthy Han children aged 0-6 years was analyzed.
  • Children were categorized into four age groups: newborn, infant, toddler, and preschool.
  • Peripheral blood samples were analyzed using flow cytometry to quantify lymphocyte subsets.

Main Results:

  • Significant sex-based differences were observed in CD3+ T cells, CD3+CD4+ T cells, CD3-CD19+ B cells, and the CD4+/CD8+ ratio.
  • Lymphocyte subset percentages and ratios showed distinct age-dependent trends, with newborns exhibiting the highest CD3+ T cell and CD3+CD4+ T cell percentages and CD4+/CD8+ ratio.
  • Natural killer (NK) cell percentages increased with age, while B cell percentages peaked in toddlers.

Conclusions:

  • The distribution of peripheral blood lymphocyte subsets in healthy children is significantly influenced by both age and sex.
  • Age- and sex-specific reference values are necessary for the accurate interpretation of lymphocyte subset data in pediatric populations.
Abstract

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