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MicroRNA-144 attenuates cardiac ischemia/reperfusion injury by targeting FOXO1

Lusha E1,2, Hong Jiang1, Zhibing Lu1

  • 1Department of Cardiology, Renmin Hospital of Wuhan University, Hubei General Hospital, Wuchang, Wuhan, Hubei 430060, P.R. China.

Experimental and Therapeutic Medicine
|February 21, 2019
PubMed

Insights

MicroRNA-144 (miR-144) is downregulated in heart ischemia/reperfusion (I/R) injury. Overexpressing miR-144 reduces myocardial injury and apoptosis, suggesting its therapeutic potential.

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Biochemistry

Background:

  • Cardiovascular ischemic diseases pose significant health risks.
  • MicroRNAs (miRs) play crucial roles in regulating cardiac injury.
  • The specific role of miR-144 in cardiac ischemia/reperfusion (I/R) injury requires further investigation.

Purpose of the Study:

  • To investigate the expression and function of miR-144 in myocardial I/R injury.
  • To identify the molecular targets of miR-144 in the context of cardiac injury.
  • To evaluate the potential of miR-144 as a therapeutic target for I/R injury.

Main Methods:

  • Reverse transcription quantitative polymerase chain reaction (RT-qPCR) for RNA expression analysis.
  • 2,3,5-triphenyltetrazolium chloride (TTC) staining to assess infarct size.
  • Terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL) assay for apoptosis detection.
  • Bioinformatic analysis (MiRanda, TargetScan) and luciferase assays to identify miR-144 targets.

Main Results:

  • miR-144 expression was significantly downregulated in a rat model of I/R injury.
  • Overexpression of miR-144 reduced myocardial infarct size and apoptosis in I/R conditions.
  • Similar protective effects of miR-144 were observed in H9c2 cells under hypoxia/reoxygenation.
  • Forkhead box protein O1 (FoxO1) was confirmed as a direct target of miR-144.

Conclusions:

  • miR-144 plays a protective role against myocardial I/R injury.
  • The miR-144/FoxO1 axis is involved in the regulation of cardiac I/R injury.
  • miR-144 holds promise as a diagnostic marker and therapeutic agent for myocardial I/R injury.
Keywords:
forkhead box protein O1ischemia/reperfusionmicroRNA-144myocardium

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