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Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
Published on: August 2, 2018
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rAAV-Mediated Gene Delivery to Adipose Tissue
Wei Huang1,2, Nicholas J Queen1,2, Lei Cao3,4
1Department of Cancer Biology and Genetics, The Ohio State University, Columbus, OH, USA.
Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2019
Summary
A novel engineered adeno-associated virus (AAV) vector, Rec2, efficiently transduces adult adipose tissue. Different administration routes target specific fat depots, offering a powerful tool for gene therapy and research.
Area of Science:
- Biotechnology
- Gene Therapy
- Adipose Tissue Biology
Background:
- Recombinant adeno-associated virus (rAAV) vectors are promising for gene therapy.
- Genetic manipulation of adult adipose tissue is hindered by low transduction efficiency of natural AAV serotypes.
Purpose of the Study:
- To evaluate a novel engineered hybrid AAV serotype, Rec2, for enhanced adipose tissue transduction.
- To compare the efficacy of Rec2 vector delivery via direct injection, oral administration, and intraperitoneal injection.
Main Methods:
- Production of Rec2 AAV vector.
- Delivery of Rec2 vector to adipose tissue via direct injection, oral administration, and intraperitoneal injection.
- Assessment of transduction efficiency and tropism in vivo.
Main Results:
- Rec2 demonstrated superior transduction of adipose tissue compared to natural AAV serotypes via direct injection.
- Oral administration of Rec2 targeted interscapular brown fat.
- Intraperitoneal injection of Rec2 preferentially targeted visceral fat.
Conclusions:
- The Rec2 vector is a potent tool for genetically manipulating adipose tissue in adults.
- Rec2 facilitates basic research and holds potential for gene therapies of metabolic diseases.
- Detailed protocols for Rec2 AAV production and delivery are provided.
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