Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphorylation01:02

Phosphorylation

53.8K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
53.8K
What is the Cell Cycle?00:56

What is the Cell Cycle?

10.5K
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the...
10.5K
What is the Cell Cycle?01:04

What is the Cell Cycle?

242.6K
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
242.6K
The Cell Cycle Control System01:28

The Cell Cycle Control System

5.6K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
5.6K
The Cell Cycle Control System02:11

The Cell Cycle Control System

14.2K
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
14.2K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

7.8K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Membrane Tubulation by Cytoskeletal System: From Physical Principles to Cellular Mechanisms and In Vitro Reconstitution.

Journal of oleo science·2026
Same author

Trends in psychotropic use among older adults with dementia in Korean long-term care hospitals across the COVID-19 pandemic: a national longitudinal study.

Frontiers in public health·2026
Same author

Therapeutic Potential of Bovine Colostrum-Derived Exosomes for Ischemia-Reperfusion Injury in Skin Flaps: A Rat Model Study.

Plastic and reconstructive surgery·2026
Same author

Kinemomics: spatiotemporal morphodynamic mapping of ventricular kinematic subpopulations in organotypic fetal heart slices.

bioRxiv : the preprint server for biology·2026
Same author

Functionalized Lipid Nanoparticles for Targeted RNA Delivery in Immune and Inflammatory Diseases.

Biomedicines·2026
Same author

Challenges and Strategies for Enhancing Nurse Managers' Financial Management Competencies: A Scoping Review.

Journal of nursing management·2026

Related Experiment Video

Updated: Jan 28, 2026

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine IdU with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
08:37

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine IdU with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

Published on: October 22, 2021

3.4K

eIF4A3 Phosphorylation by CDKs Affects NMD during the Cell Cycle.

Incheol Ryu1, You-Sub Won1, Hongseok Ha1

  • 1Creative Research Initiatives Center for Molecular Biology of Translation, Korea University, Seoul 02841, Republic of Korea; Division of Life Sciences, Korea University, Seoul 02841, Republic of Korea.

Cell Reports
|February 21, 2019
PubMed
Summary
This summary is machine-generated.

Phosphorylation of eIF4A3 by cyclin-dependent kinases regulates exon junction complex (EJC) deposition onto mRNA. This cell cycle-dependent process impacts nonsense-mediated mRNA decay (NMD) efficiency.

Keywords:
CDKcell cycleeIF4A3exon junction complexnonsense-mediated mRNA decayphosphorylation

More Related Videos

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

14.7K
Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

61.3K

Related Experiment Videos

Last Updated: Jan 28, 2026

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine IdU with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
08:37

Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine IdU with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform

Published on: October 22, 2021

3.4K
Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

14.7K
Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

61.3K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Gene Regulation

Background:

  • Exon junction complexes (EJCs) are crucial for post-transcriptional gene regulation, including nonsense-mediated mRNA decay (NMD).
  • The precise mechanism governing EJC deposition onto spliced mRNAs remains largely unelucidated.
  • eIF4A3 is a core component of the EJC, but its deposition regulation is unknown.

Purpose of the Study:

  • To investigate the regulatory mechanism controlling EJC deposition onto spliced mRNAs.
  • To determine the role of eIF4A3 phosphorylation in EJC formation and function.
  • To understand the cell cycle-dependent regulation of EJC deposition and its impact on NMD.

Main Methods:

  • Cell cycle analysis
  • Phosphorylation site mapping
  • Immunoprecipitation assays
  • RNA binding assays
  • Spliceosome assembly studies

Main Results:

  • Threonine 163 (T163) of eIF4A3 is phosphorylated by cyclin-dependent protein kinases 1 and 2 in a cell cycle-dependent manner.
  • T163 phosphorylation inhibits eIF4A3 binding to mRNA and other EJC components.
  • Phosphorylated eIF4A3 associates with CWC22, facilitating its recruitment to the spliceosome for precise EJC deposition.
  • Nonsense-mediated mRNA decay (NMD) efficiency is altered by T163 phosphorylation.

Conclusions:

  • T163 phosphorylation of eIF4A3 is a key regulatory mechanism for EJC deposition.
  • This phosphorylation event ensures accurate EJC placement relative to exon-exon junctions.
  • Cell cycle-dependent EJC deposition impacts NMD, highlighting a link between cell cycle progression and gene expression regulation.