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Updated: Jan 28, 2026

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
Published on: October 31, 2019
Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp.
Jacob Sebesta1, Allison Werner2, Christie Ann Marie Peebles3,4
1Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO, USA.
Abstract:
Synechocystis sp. PCC 6803 is a model cyanobacterium which has been investigated to produce a variety of fuels and chemicals. Genetic mutations are of interest for studying photosynthesis and engineering chemical production. Here, methods for culturing, preserving, and genetically transforming Synechocystis sp. PCC 6803 are detailed including methods to test promoter strength using the green fluorescent protein reporter. Furthermore, a method for markerless transformation of chromosomal DNA is presented. Sufficient details are provided to enable application by the novice investigator.
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