Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ions as Acids and Bases02:54

Ions as Acids and Bases

26.3K
Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
26.3K
Habitat Fragmentation02:31

Habitat Fragmentation

21.3K
Habitat fragmentation describes the division of a more extensive, continuous habitat into smaller, discontinuous areas. Human activities such as land conversion, as well as slower geological processes leading to changes in the physical environment, are the two leading causes of habitat fragmentation. The fragmentation process typically follows the same steps: perforation, dissection, fragmentation, shrinkage, and attrition.
21.3K
Common Ion Effect03:24

Common Ion Effect

46.4K
Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Châtelier’s principle. Consider the dissolution of silver iodide:
46.4K
Formation of Complex Ions03:45

Formation of Complex Ions

26.0K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.0K
Precipitation of Ions03:11

Precipitation of Ions

30.2K
Predicting Precipitation
The equation that describes the equilibrium between solid calcium carbonate and its solvated ions is:
30.2K
Ion Channels01:19

Ion Channels

91.4K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
91.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hierarchical magnetic core-shell nanoreactor (Fe<sub>3</sub>O<sub>4</sub>@SA/Trypsin@UiO-66) for efficient sensing of protease inhibitors in food and feed products.

Analytica chimica acta·2026
Same author

Association between maternal cardiovascular health assessed by modified Life's Essential 8 and risks of adverse pregnancy outcomes.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·2026
Same author

Strong interfacial electronic coupling activates NiFeOOH for alkaline seawater oxidation.

Chemical communications (Cambridge, England)·2026
Same author

Intestinal Absorption and Anti-Inflammatory Effects of a Low-Molecular-Weight α-Glucan from <i>Flammulina filiformis</i>.

Foods (Basel, Switzerland)·2026
Same author

Trait Plasticity, Resource Redirection and Strong Recovery Capacity Enhance <i>Volkameria inermis</i> Tolerance and Adaptation to Long-Term Foliar Salt Stress.

Plants (Basel, Switzerland)·2026
Same author

Exploring the Joint Impact of METS-VF and Functional Limitation on Cardiometabolic Multimorbidity Risk.

Journal of diabetes research·2026

Related Experiment Video

Updated: Jan 28, 2026

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
07:35

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Published on: December 16, 2021

2.8K

A Multiplex Fragment-Ion-Based Method for Accurate Proteome Quantification.

Jianhui Liu1,2, Yuan Zhou1,3, Yichu Shan1

  • 1CAS Key Laboratory of Separation Sciences for Analytical Chemistry , Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023 , China.

Analytical Chemistry
|February 22, 2019
PubMed
Summary

A new multiplex pseudo-isobaric dimethyl labeling (m-pIDL) method enables 6-plex proteome quantification with high accuracy. This advance overcomes limitations of previous fragment-ion-based techniques, offering improved dynamic range and reduced ratio distortion for proteomic studies.

More Related Videos

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

1.3K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.7K

Related Experiment Videos

Last Updated: Jan 28, 2026

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules
07:35

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Published on: December 16, 2021

2.8K
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

1.3K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.7K

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Molecular Biology

Background:

  • Accurate multiplex proteome quantification is crucial for understanding cellular dynamics.
  • Existing fragment-ion-based methods offer high accuracy but are limited to 3-plex capacity.
  • There is a need for methods with higher multiplexing capability and accuracy.

Purpose of the Study:

  • To develop a novel multiplex labeling method for enhanced proteome quantification.
  • To extend the multiplexing capacity of fragment-ion-based quantification.
  • To improve accuracy and reduce ratio distortion in quantitative proteomics.

Main Methods:

  • Development of multiplex pseudo-isobaric dimethyl labeling (m-pIDL).
  • Utilizing millidalton and dalton mass differences for precursor ion separation.
  • Enlarging the precursor ion isolation window to 10 m/z during data acquisition.
  • One-step dimethyl labeling strategy.

Main Results:

  • Achieved 6-plex quantification capacity with high accuracy.
  • Demonstrated high quantification accuracy within a 20-fold dynamic range.
  • Reduced ratio compression to 1.13-fold in a two-proteome model, eliminating distortions seen in other methods.
  • Showed minimal ratio variation (CV = 6.66%) among post-translational modifications.
  • Successfully applied m-pIDL to monitor TGF-β-induced epithelial-mesenchymal transition (EMT) in lung adenocarcinoma cells, identifying potential regulatory proteins.

Conclusions:

  • The m-pIDL method significantly enhances multiplexing capacity for fragment-ion-based quantification.
  • m-pIDL offers remarkable accuracy, reduced ratio distortion, and suitability for studying dynamic proteomic changes.
  • This technique opens new avenues for comprehensive proteome analysis and the development of proteomic atlases.