Related Experiment Video
Updated: Jan 28, 2026

In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
Published on: January 5, 2016
Advanced In Vitro Assay System to Measure Phosphatidylserine and Phosphatidylethanolamine Transport at
Yasushi Tamura1, Rieko Kojima2, Toshiya Endo3
1Faculty of Science, Yamagata University, Yamagata, Japan. tamura@sci.kj.yamagata-u.ac.jp.
Abstract:
A number of previous studies have shown that phospholipid molecules come and go between the endoplasmic reticulum (ER) and mitochondrial membranes while the molecular basis of non-vesicular phospholipid transport is still not understood well. In this chapter, we describe an optimized method that uses membrane fractions isolated from yeast cells to directly analyze phospholipid transport between the ER and mitochondria. With this assay, we are able to assess not only the ER-to-mitochondria but also mitochondria-to-ER transports at the same time. We believe that this assay system can accelerate the research on inter-organelle phospholipid trafficking.
Related Concept Videos
Peroxisomes and Mitochondria
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
Electron Transport Chains
The ETC is comprised of...
Mitochondria
Protein-protein Interfaces
Facilitated Transport
Primary Active Transport

