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Measurement of Intracellular Sterol Transport in Yeast

Neha Chauhan1, Julian A Jentsch1,2, Anant K Menon3

  • 1Department of Biochemistry, Weill Cornell Medical College, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2019
PubMed

Keywords:
CholesterolDehydroergosterolDetergent-resistant membranesEndoplasmic reticulumErgosterolFluorescence microscopyFörster resonance energy transfer (FRET)Lipid dropletsLiposomeMethyl-β-cyclodextrinPlasma membraneSubcellular fractionationAcyl-CoA:sterol acyltransferase (ACAT)Intracellular transportYeast sterolMembrane transportLipid homeostasis

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