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Nanoparticle physicochemical properties determine the activation of intracellular complement
Anna N Ilinskaya1, Ankit Shah1, Alan E Enciso2
1Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD.
Nanomedicine : Nanotechnology, Biology, and Medicine
|February 23, 2019
Summary
Cationic polymeric nanoparticles activate intracellular complement (IC) in T-cells by damaging cell membranes. This highlights IC
Area of Science:
- Immunology
- Nanotechnology
- Cell Biology
Background:
- The complement system is crucial for immunity, traditionally involving plasma proteins.
- Immune cells like T-cells also express intracellular complement (IC), regulating T-cell activation.
- The interaction between nanomaterials and IC is not well understood.
Purpose of the Study:
- To investigate the structure-activity relationship between nanomaterials and intracellular complement (IC) in T-cells.
- To determine the mechanisms by which nanomaterials interact with IC.
- To identify factors influencing IC activation by nanomaterials.
Main Methods:
- In vitro studies using various nanomaterials with T-cells.
- Analysis of IC activation based on material properties like surface charge and size.
- Investigation of the cellular mechanisms involved in IC activation.
Main Results:
- Only polymeric nanomaterials with cationic surfaces activated IC in T-cells under in vitro conditions.
- IC activation was dependent on particle size.
- The mechanism involved membrane damage, with IC acting as a self-opsonization marker.
Conclusions:
- Cationic polymeric nanomaterials can activate intracellular complement (IC) in T-cells.
- Nanoparticle surface properties (charge, size) and membrane damage are key factors in IC activation.
- IC on the cell surface may serve as an indicator of nanoparticle-induced cellular danger.
Keywords:
AnaphylatoxinsCARPAComplementHypersensitivityICIn vitroInfusion reactionsNanoparticlesPreclinicalT-cellsMore Related Videos
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