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Updated: Jan 28, 2026

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
Published on: October 9, 2021
Estimation of Tryptophan Content by Spectrophotometric Methods: Analysis of the Interaction Between
N E Anderson1, F M Clydesdale1
1Department of Food Science and Nutrition University of Massachusetts Amherst, Massachusetts 01003.
Abstract:
The interaction between 1-tryptophan and a-ketoglutaric acid (a-KGA), first reported by Chu and Clydesdale (1,2), was used as the basis for development of a method for estimation of tryptophan content. Analysis of reaction mixtures with ultraviolet spectrophotometers revealed the development of absorbance with a wavelength of maximum absorbance (λ max) at 358 nm. Four parameters were manipulated to increase the rate and amount of chromophore formed. These parameters were: concentration of HCI, concentration of a-KGA, concentration of sodium nitrite, and temperature.
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