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Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
Recovery of Salmonellae from Broiler Carcasses by Direct Enrichment
1Animal Products Laboratory, Richard B. Russell Agricultural Research Center, United States Department of Agriculture, Agricultural Research Service, Athens, Georgia 30604.
Abstract:
Each of four serotypes of Salmonella ( S. anatum , S. montevideo , S. saint-paul , S. typhimurium ), inoculated at low levels on broiler carcasses (ca. 20 cells/carcass) was detected by direct enrichment of the whole carcass rinse fluid with either Selenite Cystine Broth (SC) or Selenite Brilliant Green Broth (SGB). Neither Selenite Brilliant Green Sulfa Broth (SBGS) nor TT Broth was effective in detecting the serotypes unless the entire broiler carcass with the rinse fluid was incubated with either of these enrichment broths. SBG and SC were effective as direct enrichment broths for recovering pure cultures of the four seroytpes subjected to sublethal heat treatment (53 C for 1 min) approximating that to which broiler carcasses are subjected during the commercial scalding process.
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