Related Experiment Video
Updated: Jan 28, 2026
09:35
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
13.9K
First Report of Cucumber leaf spot virus in Poland
H Pospieszny1, M Cajza1, R Plewa2
1Institute of Plant Protection, Department of Virology and Bacteriology, Miczurina 20, 60-318 Poznań, Poland.
Plant Disease
|February 24, 2019
Summary
Cucumber leaf spot virus (CLSV) was identified in Poland for the first time, affecting cucumber plants with stunting and leaf spots. Molecular analysis confirmed the CLSV presence, though it appears to pose no significant threat to Polish cucumber crops currently.
Area of Science:
- Plant Pathology
- Virology
- Molecular Biology
Background:
- Cucumber plants in Poland exhibited stunting and leaf spotting.
- Symptoms were transient, making initial diagnosis challenging.
Purpose of the Study:
- To identify and characterize the virus causing symptoms in Polish cucumber plants.
- To determine the genetic relationship of the identified virus to known isolates.
Main Methods:
- Mechanical inoculation of various plant species to assess host range.
- Electron microscopy for virus particle visualization.
- Reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of the RNA-dependent RNA polymerase gene.
Main Results:
- The virus caused local and systemic infections in cucumber and Nicotiana species, and local lesions in several other species.
- Electron microscopy revealed spherical virus particles approximately 30 nm in diameter.
- RT-PCR and sequence analysis confirmed the virus as Cucumber leaf spot virus (CLSV), with high nucleotide and amino acid identity to other CLSV isolates.
Conclusions:
- This study reports the first occurrence of CLSV in Poland.
- The identified CLSV isolate is genetically similar to other European and Spanish isolates.
- CLSV does not appear to be a significant threat to cucumber production in Poland based on its incidental occurrence.
Related Concept Videos
00:50What are Viruses?
128.0K
Overview
128.0K
01:24Data Reporting and Recording
5.4K
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
5.4K
01:25Types of Reports I: Hands-off Report
1.5K
A hand-off report, also known as a change-of-shift report, is a crucial nursing process that ensures the smooth transition of patient care responsibilities between nursing staff.
Following are the key components and categories of hand-off reports:
Purpose and Process:
Following are the key components and categories of hand-off reports:
Purpose and Process:
1.5K
01:21Types of Reports II: Incident or Occurrence Report
1.2K
An Incident or Occurrence Report in a healthcare setting is a crucial document used to record any unexpected occurrence that may or may not have affected a patient, employee, or visitor. Such reports are critical to improving patient safety and include all details leading up to and including the event.
Purposes:
In the healthcare industry, reports play a crucial role in documenting incidents within an agency. The primary objective of these reports is to ensure patient safety, uphold the...
Purposes:
In the healthcare industry, reports play a crucial role in documenting incidents within an agency. The primary objective of these reports is to ensure patient safety, uphold the...
1.2K
01:26Types of Reports III: Telephone and Verbal Reports
1.0K
Telephone and Verbal Reports in healthcare settings are two communication methods for conveying therapeutic instructions from healthcare providers to nurses or other healthcare staff.
Here's an overview of each type:
Telephone Orders
Here's an overview of each type:
Telephone Orders
1.0K
02:11Reporter Genes
13.2K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.2K