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Updated: Jan 28, 2026
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
Published on: March 3, 2015
10.0K
Functional assays for transcription mechanisms in high-throughput
1Department of Medicine, Division of Translational Therapeutics, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA; Cancer Research Institute, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Methods (San Diego, Calif.)
|February 24, 2019
Summary
High-throughput sequencing and functional assays accelerate discoveries in nucleic acid and protein biology. These methods offer valuable insights into biological systems, exemplified by studies of the RNA Polymerase II active site.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- Advances in nucleic acid and protein biology are driven by large-scale programmed synthesis of nucleic acid libraries and deep sequencing.
- High-throughput studies are crucial for biological systems involving nucleic acids or encoded proteins, enabling analysis of synthesized DNA variant pools and transcription products.
Purpose of the Study:
- To review the scope of high-throughput functional assays for nucleic acid and protein studies.
- To discuss how these studies provide insights into biological systems, using the RNA Polymerase II active site as a case study.
- To highlight methodological considerations for designing and executing such experiments.
Main Methods:
- Programmed synthesis of large-scale nucleic acid libraries.
- Deep sequencing for analyzing DNA, RNA, and protein products.
- High-throughput functional assays tailored for nucleic acids and proteins.
Main Results:
- High-throughput methods have significantly advanced the understanding of nucleic acid and protein biology.
- Studies utilizing these techniques have provided key insights into the RNA Polymerase II active site.
- Methodological considerations are crucial for successful experimental design and execution.
Conclusions:
- High-throughput functional assays and deep sequencing are powerful tools for biological research.
- These approaches offer broad applicability across various biological systems.
- Careful consideration of experimental design is essential for maximizing the value of high-throughput studies.
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