Two symmetrical unsaturated acids isolated from Viscum album

Area of Science:

• Natural Product Chemistry
• Medicinal Chemistry
• Phytochemistry

Background:

• Viscum album (mistletoe) is a plant with a history of medicinal use.
• Acetylene conjugate compounds are a class of organic molecules with diverse biological activities.
• Oxidative stress is implicated in various diseases, making antioxidants a key area of research.

Purpose of the Study:

• To isolate and characterize new acetylene conjugate compounds from Viscum album.
• To evaluate the antioxidant potential of isolated compounds.
• To identify novel compounds with therapeutic applications.

Main Methods:

• Extraction and isolation of compounds from dry stem leaves of Viscum album.
• Structure elucidation using comprehensive spectroscopic data (e.g., NMR, MS).
• In vitro antioxidant assays, including inhibition of xanthine oxidase (XOD) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging.

Main Results:

• Two new acetylene conjugate compounds, dibutyl (2Z, 6Z)-octa-2, 6-dien-4-yne dioate (1) and dibutyl (2E, 6E)-octa-2, 6-dien-4-yne dioate (2), were successfully isolated and characterized.
• Compounds 1 and a known compound (8) exhibited notable antioxidant activity.
• Compound 1 showed potent inhibition against xanthine oxidase (IC50: 1.22 μmol·L-1) and DPPH radical scavenging (SC50: 4.34 μmol·L-1).

Conclusions:

• Viscum album is a source of novel acetylene conjugate compounds with significant antioxidant properties.
• Compound 1 is a promising candidate for further investigation as a natural antioxidant.
• The study contributes to understanding the chemical constituents and bioactivity of Viscum album.