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CRISPR/Cas9-Assisted Genome Editing in Murine Embryonic Stem Cells
Artiom Gruzdev1, Greg J Scott2, Thomas B Hagler2
1Knockout Mouse Core, Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA. artiom.gruzdev@nih.gov.
Methods in Molecular Biology (Clifton, N.J.)
|February 25, 2019
Summary
Genetically modified mouse models, utilizing CRISPR/Cas9 technology, help overcome human genetic diversity challenges. This enables precise gene function studies for advancing human health and disease research.
Area of Science:
- Genetics
- Genomics
- Molecular Biology
Background:
- Human gene function studies are complex due to genetic diversity and environmental factors.
- Inbred mouse models offer controlled environments to study gene function and disease.
- Genetically modified mice allow for precise genetic alterations to investigate gene roles.
Purpose of the Study:
- To explore the utility of genetically modified mouse models in understanding gene function.
- To highlight the impact of CRISPR/Cas9 genome editing in creating these models.
- To provide insights into human health and disease through murine models.
Main Methods:
- Utilizing inbred mouse lines to control for non-genomic variables.
- Generating genetically modified mouse models with specific mutations.
- Employing CRISPR/Cas9 genome editing in murine embryonic stem cells.
Main Results:
- Genetically modified mouse models facilitate the elucidation of essential gene functions.
- CRISPR/Cas9 enables efficient and precise genome editing in mice.
- These models help isolate the impact of specific genetic changes.
Conclusions:
- Genetically modified mouse models are crucial for dissecting gene function in complex biological systems.
- CRISPR/Cas9 technology significantly advances the creation of these valuable research tools.
- Understanding gene function in mice provides critical insights into human physiology and pathophysiology.
Keywords:
Blastocyst isolationCRISPR/Cas9Chimeric miceEmbryonic stem cell screeningEmbryonic stem cell transfectionEmbryonic stem cellsGene-targetingGenetically modified miceGermline transmissionMicroinjectionMore Related Videos
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