Related Experiment Video
Updated: Jan 28, 2026
07:44
Design and Synthesis of a Reconfigurable DNA Accordion Rack
Published on: August 15, 2018
7.5K
Design, Synthesis, and Actions of an Innovative Bispecific Designer Peptide
Laura M G Meems1,2, Ingrid A Andersen1,2, Shuchong Pan1,2
1From the Cardiorenal Research Laboratory (L.M.G.M., I.A.A., S.P., G.H., Y.C., Y.Z., G.E.H., T.I., D.M.H., S.R.I., S.J.S., J.C.B.), Mayo Clinic, Rochester, MN.
Hypertension (Dallas, Tex. : 1979)
|February 26, 2019
Summary
A novel bispecific peptide, NPA7, simultaneously targets two key receptors to offer enhanced cardiorenal protective benefits. This designer peptide shows promise as a new therapeutic for cardiovascular diseases like heart failure and hypertension.
Area of Science:
- Cardiovascular Research
- Peptide Engineering
- Pharmacology
Background:
- Cardiovascular disease is the leading cause of global mortality despite current treatments.
- Peptide engineering offers innovative therapeutic strategies for various diseases.
- Bispecific therapeutics targeting multiple signaling pathways are a novel approach for cardiovascular disease.
Purpose of the Study:
- To engineer and characterize a novel bispecific peptide, NPA7, targeting both the particulate guanylate cyclase A (pGC-A) receptor and the Mas receptor (MasR).
- To evaluate the in vitro and in vivo efficacy of NPA7 as a potential cardiorenoprotective therapeutic for cardiovascular disease.
Main Methods:
- NPA7 was engineered by fusing a B-type natriuretic peptide (BNP) sequence with Angiotensin 1-7 (Ang 1-7).
- In vitro studies assessed NPA7's dual receptor activation via second messenger production and receptor interaction.
- In vivo studies in canines evaluated NPA7's biological actions, including its response to an MasR antagonist.
Main Results:
- Successful synthesis of NPA7, a bispecific activator for pGC-A and MasR, was confirmed.
- In canines, NPA7 demonstrated enhanced natriuretic, diuretic, systemic, and renal vasorelaxing properties.
- NPA7 exhibited superior effects compared to individual native ligands for pGC-A and MasR.
Conclusions:
- NPA7 is a novel bispecific designer peptide with demonstrated dual receptor-activating capabilities.
- NPA7 shows significant potential as a cardiorenal therapeutic agent for cardiovascular diseases, including hypertension and heart failure.
Related Concept Videos
02:01Group Design
10.4K
The most basic experimental design involves two groups: the experimental group and the control group. The two groups are designed to be the same except for one difference— experimental manipulation. The experimental group gets the experimental manipulation—that is, the treatment or variable being tested—and the control group does not. Since experimental manipulation is the only difference between the experimental and control groups, we can be sure that any differences between...
10.4K
02:01Factorial Design
13.8K
Factorial Analysis is an experimental design that applies Analysis of Variance (ANOVA) statistical procedures to examine a change in a dependent variable due to more than one independent variable, also known as factors. Changes in worker productivity can be reasoned, for example, to be influenced by salary and other conditions, such as skill level. One way to test this hypothesis is by categorizing salary into three levels (low, moderate, and high) and skills sets into two levels (entry level...
13.8K
01:25Design Example: Designing a Residential Plumbing System
1.1K
The design of residential plumbing systems requires carefully evaluating water demand, flow rates, and pressure dynamics to ensure both efficiency and reliability. The nature of water flow within pipes is defined by its Reynolds number, which classifies flow as either laminar (smooth) or turbulent.
1.1K
01:18Design Example: Designing Water Slide
625
When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed.
Bernoulli's principle determines the water's velocity along the slide....
Bernoulli's principle determines the water's velocity along the slide....
625
01:27Design Example: Design of an Irrigation Channel
824
Trapezoidal channels are widely used in irrigation systems due to their cost-effectiveness and efficiency in conveying water. Trapezoidal channels feature a flat bottom and sloping sides, making them stable and easier to construct compared to other shapes. The bottom width and side slope ratio are determined based on the required flow capacity and site conditions. The side slope is kept gentle for unlined channels to prevent soil erosion.Hydraulic parameters in channel design include the flow...
824
01:23Design Example
552
The innovation of touch-tone telephony revolutionized the telecommunications industry by replacing the traditional rotary dial with a dual-tone multi-frequency (DTMF) signaling system. This system uses a matrix-style keypad with buttons arranged in four rows and three columns, creating 12 distinct signals each assigned to a pair of frequencies. Each button press results in a simultaneous generation of two sinusoidal tones – one from a low-frequency group (697 to 941 Hz) and one from a...
552